Differential Expression of Genes Associated with Lipid Metabolism in Diapausing Lysiphlebus testaceipes Cresson

doi:10.16409/j.cnki.2095-039x.2020.03.022

Abstract

Abstract: Lysiphlebus testaceipes Cresson (Hymenoptera:Aphidiidae) is an excellent ectoparasitic wasp of Aphis craccivora Koch. This parasitoid has the potential to suppress populations of A. craccivora. Diapause is a genetic phenomenon of insects adapting to unfavorable environmental conditions. In this study, we employed transcriptome sequencing analyses to screen differentially expressed genes (DEGs) between diapause groups and non-diapause groups. We found that the DEGs were primarily associated with carbohydrate metabolism, lipid metabolism, signal transduction and so on. With the help of KEGG database, a total of 401 DEGs related to lipid metabolism during diapause were screened out. These DEGs, up-regulated to some extent during diapause, are related to fatty acid biosynthesis, glycerol metabolism, steroid biosynthesis and other metabolic pathways. Expression of the genes related to fatty acid synthase and elongase of verylong chain fatty acids was up-regulated, genes of enzymes related to lipid decomposition, such as esterase, were also up-regulated in expression, which may be involved in immunity improvement in L. testaceipes. The up-regulated expression of genes related to hormone synthesis may inhibit the ovarian development.

Key words: Lysiphlebus testaceipes, transcriptomics, diapause-associated gene, lipid metabolism

CLC Number:

S476.3

LIU Min, LIU Aiping, HAN Haibin, GAO Shujing, XU Linbo, YUE Fangzheng, HUANG Haiguang. Differential Expression of Genes Associated with Lipid Metabolism in Diapausing Lysiphlebus testaceipes Cresson[J]. Chinese Journal Of Biological Control, 2020, 36(4): 632-636.

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