Differential Expression of Proteins Associated with Lipid Metabolism in Diapausing Aphidius gifuensis Ashmaed

doi:10.16409/j.cnki.2095-039x.2015.06.001

Abstract

Abstract: Diapause may contribute to the shelf life of mass-produced Aphidius gifuensis. To better understand the diapause mechanism of A. gifuensis, iTRAQ quantitative techniques combined with bioinformatics were used to analyze differentially expressed proteins between non-diapausing and diapausing A. gifuensis. A total of 1268 proteins were quantitatively identified and 601 of them were differentially expressed, with 278 up-regulated proteins and 323 down-regulated proteins. There were 4 prominently up-regulated proteins in diapausing A. gifuensis, which were associated with lipid metabolism: hypothetical protein SINV_00606, partial (gi_322794737), conserved uncharacterized protein (gi_607305114), peptidyl-prolyl cis-trans isomerase (W4X351_ATTCE), arylphorin subunit alpha (gi_332018934). These results showed that iTRAQ technique combined with LC-MS/MS is applicable to protein quantification in diapausing A. gifuensis. The results laid a foundation for function analysis of the significantly up-regulated proteins and for further study of the diapausing mechanism in insects.

Key words: diapause, Aphidius gifuensis, iTRAQ quantitative techniques, diapause associated protein, lipid metabolism

CLC Number:

S476.3

HUANG Fengxia, JIANG Sha, REN Xiaoyun, AN Tao, CHEN Hongyin, ZHANG Lisheng. Differential Expression of Proteins Associated with Lipid Metabolism in Diapausing Aphidius gifuensis Ashmaed[J]. journal1, 2015, 31(6): 811-820.

References

[1] 张礼生. 滞育和休眠在昆虫饲养中的应用//曾凡荣, 陈红印主编. 天敌昆虫饲养系统工程[M]. 北京: 中国农业科学技术出版社, 2009, 54-89.
[2] 张礼生. 蚜茧蜂的扩繁与应用//张礼生, 陈红印, 李保平主编. 天敌昆虫扩繁与应用[M]. 北京: 中国农业科学技术出版社, 2014, 148-169.
[3] Denlinger D L, Armbruster P A. Mosquito diapause[J]. Annual Review of Entomology, 2014, 59: 73-93.
[4] Hahn D A, Denlinger D L. Energetics of insect diapause[J]. Annual Review of Entomology, 2011, 56: 103-121.
[5] Christiea A E, Roncallia V, Lonab P B, et al. In silico characterization of the insect diapause-associated protein couch potato (CPO) in Calanus finmarchicus (Crustacea: Copepoda)[J]. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 2013, 8(1): 45-57.
[6] 刘遥, 张礼生, 陈红印, 等. 苹果酸脱氢酶与异柠檬酸脱氢酶在滞育七星瓢虫中的差异表达[J]. 中国生物防治学报, 2014, 30(5): 593-599.
[7] Richard D U, John R G, Anthony D W, et al. Simultaneous analysis of relative protein expression levels across multiple samples using iTRAQ isobaric tags with 2D nano LC–MS/MS[J]. Nature Protocols Erecipes for Researchers, 2010, 5(9): 1574-1582.
[8] White F M. On the iTRAQ of kinase inhibitors[J]. Nature Biotechnology, 2007, 25(9): 994-996.
[9] Karp N A, Huber W, Pawel G S, et al. Addressing accuracy and precision issues in iTRAQ quantitation[J]. Molecular and Cellular Proteomics, 2010, 9(9): 1885-1897.
[10] 黄凤霞, 周阳, 张礼生, 等. 一种扩繁烟蚜茧蜂的载体植物适合性评价[J]. 环境昆虫学报, 2015, 37(4) : 827-833.
[11] 李玉艳, 张礼生, 陈红印, 等. 烟蚜茧蜂滞育诱导的温光周期反应[J]. 应用昆虫学报, 2013, 50(3): 718-726.
[12] 张礼生, 陈红印, 王孟卿, 等. 寄生蜂的滞育研究进展[J]. 中国生物防治学报, 2014, 30(2): 149-164.
[13] 徐珊珊, 阎春兰, 刘黎明, 等. 细胞裂解液对蛋白质定量方法的影响[J]. 浙江大学学报(医学版), 2008, 37(1): 45-50.
[14] 胡文霞. 葱蝇非滞育和夏滞育蛹的比较蛋白质组学研究[D]. 重庆: 重庆师范大学, 2011.
[15] Benjamini Y, Hochberg Y. Controlling the false discovery rate: a practical and powerful approach to multiple testing[J]. Journal of the Royal Statistical Society Series B: Statistical Methodology, 1995, 57: 289-300.
[16] Wu W W, Wang G, Baek S J, et al. Comparative study of three proteomic quantitative methods, DIGE, ICAT, and iTRAQ, using 2D Gel or LC- MALDI TOF/TOF[J]. Journal of Proteome Research, 2006, 5(3): 651-658.
[17] Philipp M, Namrata D U, Karl R C, et al. iTRAQ labeling is superior to mTRAQ for quantitative global proteomics and phosphoproteomics[J]. Molecular and Cellular Proteomics, 2012, 11(6): M111.008466.
[18] Ranju R, DeSouza L V, Matta A, et al. Discovery and verification of head-and-neck cancer biomarkers by differential protein expression analysis using iTRAQ labeling, multidimensional liquid chromatography, and tandem mass spectrometry[J]. Molecular and Cellular Proteomics, 2008, 7(6): 1162-1173.
[19] Wolff S, Otto A, Albrecht D, et al. Gel- free and gel- based proteomics in Bacillus subtilis: A comparative study [J]. Molecular and Cellular Proteomics. 2006, 5(7): 1183-1192.
[20] Golovan S P, Hakimov H A, Verschoor C P, et al. Analysis of Sus scrofa liver proteome and identification of proteins differentially expressed between genders, and conventional and genetically enhanced lines, and conventional and genetically enhanced lines[J]. Comparative Biochemistry and Physiology Part D, Genomics and Proteomics, 2008, 3(3): 234-242.
[21] Zhu M, Simons B, Zhu N, et al. Analysis of abscisic acid responsive proteins in Brassica napus guard cells by multiplexed isobaric tagging[J]. Journal of Proteomics, 2010, 73(4): 790-805.
[22] Zhu J Y, Wu G X, Ze S Z, et al. Parasitization by Scleroderma guani influences protein expression in Tenebrio molitor pupae[J]. Archives of Insect Biochemistry and Physiology, 2014, 66: 37-44.
[23] 李玉艳. 烟蚜茧蜂滞育诱导的温光周期反应及滞育生理研究[D]. 北京: 中国农业科学院, 2011.
[24] 张洁, 张礼生, 陈红印, 等. 大规模扩繁烟蚜茧蜂的蚜类寄主筛选研究[J]. 中国生物防治报, 2014, 30(1): 32-37.
[25] 黄继梅. 烟蚜茧蜂防治烟蚜研究及推广应用[D]. 长沙: 湖南农业大学, 2008.
[26] Denlinger D L. Why study diapause[J]. Entomological Research, 2008, 38(1): 1- 9.
[27] 张礼生, 陈红印. 生物防治作用物研发与应用的进展[J]. 中国生物防治学报, 2014, 30(5): 581-586.
[28] Denlinger D L. Regulation of diapause[J]. Annual Review of Entomology, 2002, 47(1): 93-122.
[29] Hahn D A, Denlinger D L. Meeting the energetic demands of insect diapause: nutrient storage and utilization[J]. Journal of Insect Physiology, 2007, 53(8): 760-773.
[30] Li A Q, Popova B A, Dean D H, et al. Proteomics of the flesh fly brain reveals an abundance of upregulated heat shock proteins during pupal diapause[J]. Journal of Insect Physiology, 2007, 53(4): 385-391.
[31] Veerkamp J H, Zimmerman A W. Fatty acid-binding proteins of nervous tissue[J]. Journal of Molecular Neuroscience, 2001, 16(2-3): 133-142.
[32] 王丹, 唐田娟, 刘悦, 等. 家蚕亲环蛋白A基因(BmCyPA)的克隆与表达分析[J]. 蚕业科学, 2011, 37(5): 792-800.
[33] Brown J J, Chippendale G M. Juvenile hormone and a protein associated with the larval diapause of the southwestern corn borer, Diatraea grandiosella[J]. Insect Biochemistry, 1978, 8(5): 359-367.
[34] Sim C, Denlinger D L. Insulin signaling and FOXO regulate the overwintering diapause of the mosquito Culex pipiens[J]. PNAS, 2008, 105(18): 6777-6781.
[35] Sim C, Denlinger D L. Juvenile hormone III suppresses forkhead of transcription factor in the fat body and reduces fat accumulation in the diapausing mosquito, Culex pipiens[J]. Insect Molecular Biology, 2013, 22(1): 1-11.