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journal1 ›› 2015, Vol. 31 ›› Issue (6): 811-820.DOI: 10.16409/j.cnki.2095-039x.2015.06.001

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Differential Expression of Proteins Associated with Lipid Metabolism in Diapausing Aphidius gifuensis Ashmaed

HUANG Fengxia1, JIANG Sha1,2, REN Xiaoyun1, AN Tao1, CHEN Hongyin1, ZHANG Lisheng1   

  1. 1. Sino-American Biological Control Laboratory/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
    2. College of Plant Protection, Fujian Agricultural and Forestry University, Fuzhou 350002, China
  • Received:2015-01-16 Online:2015-12-08 Published:2015-12-17

Abstract: Diapause may contribute to the shelf life of mass-produced Aphidius gifuensis. To better understand the diapause mechanism of A. gifuensis, iTRAQ quantitative techniques combined with bioinformatics were used to analyze differentially expressed proteins between non-diapausing and diapausing A. gifuensis. A total of 1268 proteins were quantitatively identified and 601 of them were differentially expressed, with 278 up-regulated proteins and 323 down-regulated proteins. There were 4 prominently up-regulated proteins in diapausing A. gifuensis, which were associated with lipid metabolism: hypothetical protein SINV_00606, partial (gi_322794737), conserved uncharacterized protein (gi_607305114), peptidyl-prolyl cis-trans isomerase (W4X351_ATTCE), arylphorin subunit alpha (gi_332018934). These results showed that iTRAQ technique combined with LC-MS/MS is applicable to protein quantification in diapausing A. gifuensis. The results laid a foundation for function analysis of the significantly up-regulated proteins and for further study of the diapausing mechanism in insects.

Key words: diapause, Aphidius gifuensis, iTRAQ quantitative techniques, diapause associated protein, lipid metabolism

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