Optimization of Oviposition Conditions for Artificial Rearing of Spodoptera litura

doi:10.16409/j.cnki.2095-039x.2023.01.019

Abstract

Abstract: Spodoptera litura (Fabricius) egg is a dominant alternative host of Telenomus remus (Nixon), a promising parasitoid of pest in China. High oviposition rate and availability of S. litura eggs are significant for improving the large-scale multiplication and application of T. remus. Therefore, the oviposition preference of S. litura for 4 substrates, 6 colors, 3 positions, and illumination conditions was investigated. The results showed that the preference of S. litura for egg-laying substrates ranked from high to low was filter paper＞non-woven fabric＞copy paper＞gauze. The total number of eggs and oviposition rate on filter paper were the highest of 2266 eggs/female and 46.3%, respectively. Although the females showed no preference for colors, they preferred to oviposit on the back of the substrate. The mean number of eggs and oviposition rate on the substrate back were 4435.17 eggs/female and 92.4%, respectively, which were significantly higher than those on each of the side and the front of the substrate. In addition, compared with dark conditions, females prefer to lay eggs under bright conditions, as evidenced by higher fecundity (2352.8 eggs/female) and oviposition selection rate (71.2%) at bright than dark conditions. Collectively, these results prove that the oviposition substrate, location, and illumination conditions can significantly affect the oviposition of S. litura, which can support the development of techniques for improving mass-production and commercial application of T. remus in biological pest control.

Key words: Spodoptera litura, oviposition preference, scale expansion, oviposition substrate, ovipoisition location, illumination conditions

CLC Number:

S476

JING Xiaoyu, ZHOU Lei, ZHANG Hongzhi, CHEN Wanbin, LI Yuyan, MAO Jianjun, WANG Mengqing, ZHANG Lisheng. Optimization of Oviposition Conditions for Artificial Rearing of Spodoptera litura[J]. Chinese Journal of Biological Control, 2023, 39(6): 1301-1308.

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