Secondary Metabolites of Bacillus cereus SAU03 and Their Nematicidal Activities

doi:10.16409/j.cnki.2095-039x.2025.02.008

Abstract

Abstract: This study aimed to isolate and screen bacterial strains with nematicidal activity from food waste degradation products and to elucidate the structures and classifications of secondary metabolites produced by the active strains. Bacteria were isolated from food waste degradation products using the heat strike dilution method, and active strains producing abundant secondary metabolites were screened by high performance liquid chromatography (HPLC) combined with the immersion method. Candidate strains were further characterized through molecular biology techniques, while chromatographic and spectroscopic methods were employed to purify and identify the structures of the isolated compounds. The nematicidal activity of the isolated compounds was also evaluated. A total of 10 morphologically distinct bacterial strains were isolated, among which strain SAU03 exhibited the highest abundance of secondary metabolites and the strongest nematicidal activity. Based on the 16S rDNA sequence analysis, strain SAU03 was identified as Bacillus cereus. Nine compounds were isolated and characterized from strain SAU03, including one new diaryl ether compound 2-(4-(4-(2-Methoxy-2-oxoethyl) phenoxy) phenyl) acetic acid (1), and eight known compounds. The known compounds were N-acetyltyramine (2), cyclo-(L-4-Hyp-L-Phe) (3), cyclo-(L- Phe-cis-4-hydroxy-D-Pro) (4), cyclo-(L-4-Hyp-L-Leu) (5), cyclo-(Gly-L-Pro) (6), phenylacetic acid (7), anthranilic acid (8), and thymine (9), respectively. Among these, compound 8 exhibited the most potent nematicidal activity, demonstrating superior inhibition of Bursaphelenchus xylophilus compared to the positive control, rotenone, at both 24 and 36 hours under identical treatment conditions. This study provides valuable insights for the development and utilization of microbial resources and the discovery of nematicidal agents.

Key words: Bacillus cereus, food waste degradation products, secondary metabolites, nematicidal activity

CLC Number:

Q939.9

LIU Zewen, CAI Teng, YANG Yuting, CHEN Shiqi, LI Yitong, LIU Tianyi, WANG Honghong, MAO Ziling, SHAN Tijiang. Secondary Metabolites of Bacillus cereus SAU03 and Their Nematicidal Activities[J]. Chinese Journal of Biological Control, 2025, 41(1): 91-102.

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