Antifungal Activity and Mechanism of an Endophytic Bacterium ZY-1 from Wheat Root against Blumerial graminis f. sp. Tritici

doi:10.16409/j.cnki.2095-039x.2016.05.013

Abstract

Abstract: Six Bacillus strains isolated from wheat roots were evaluated for antifungal activity using oil-spreading and inhibitory tests. One strain, ZY-1, was found to have higher activity than the others, and PCR analysis revealed that it contained at least four antifungal genes including srfA, fenA, ituB, and bacD. Phylogenetic analysis of its 16S rDNA gene revealed that ZY-1 was a new strain of Bacillus cereus. Further investigation using in vitro plate assays and greenhouse experiments revealed that strain ZY-1 provided better control on Blumeria graminis f. sp. tritici than 10 mg/L applications of the chemical fungicide triadimefon, with control efficacies of 97.94%±1.25% and 82.56%±2.11%, respectively (P<0.05). In vivo histological analysis of the culture filtrate showed strain ZY-1 had a dramatic effect on the development of B. graminis, inhibiting both the germination of conidia and the formation of haustoria (P<0.05). Taken together, these results indicated that the lipopeptide-producing B. cereus stain ZY-1 has great potential as a biological control agent against B. graminis.

Key words: Bacillus cereus, antifungal activity, mechanism, Blumeria graminis f. sp. Tritici

CLC Number:

S476

GONG Shuangjun, XIANG Libo, XUE Minfeng, ZHANG Xuejiang, SHI Wenqi, ZENG Fansong, WANG Hua, YANG Lijun, YU Dazhao. Antifungal Activity and Mechanism of an Endophytic Bacterium ZY-1 from Wheat Root against Blumerial graminis f. sp. Tritici[J]. journal1, 2016, 32(5): 635-641.

References

[1] Zheng Y, Luo Y, Zhou Y, et al. Real-time PCR quantification of latent infection of wheat powdery mildew in the field[J]. European Journal of Plant Pathology, 2013, 136(3):565-575.
[2] 邱德文. 生物农药研究进展与未来展望[J]. 植物保护, 2013, 39(5):81-89.
[3] Paulitz T C,Be'langer R R. Biological control in greenhouse systems[J]. Annual Review of Phytopathology, 2001, 39(1):103-133.
[4] Be'langer R R, Benyagoub M. Challenges and prospects for integrated control of powdery mildews in the greenhouse[J]. Canadian Journal of Plant Pathology, 1997, 19(3):310-314.
[5] Elad Y, Malathrakis N E, Dik A J. Biological control of Botrytis-incited diseases and powdery mildews in greenhouse crops[J]. Crop Protection, 1996, 15(3):229-240.
[6] Romero D, P'erez-Garc'ıa A, Rivera M E, et al. Isolation and evaluation of antagonistic bacteria towards the cucurbit powdery mildew fungus Podosphaera fusca[J]. Applied Microbiology and Biotechnology, 2004, 64(2):263-269.
[7] Li Y L, Gu Y L, Li J, et al. Biocontrol agent Bacillus amyloliquefaciens LJ02 induces systemic resistance against cucurbits powdery mildew[J]. Frontiers in Microbiology, 2015, (6):833.
[8] Belanger R R, Labbe C, Jarvis W. Commercial-scale control of rose powdery mildew with a fungal antagonist[J]. Plant Disease, 1994, 78(4):420-424.
[9] Urquhart E J, Menzies J G, Punja Z. Growth and biological control activity of Tilletiopsis species against powdery mildew (Sphaerotheca fuliginea) on greenhouse cucumber[J]. Phytopathology, 1994, 84(4):341-351.
[10] 朱震, 罗毅, 张鹏, 等. 产表面活性素和伊枯草菌素A菌株的筛选及其脂肽类产物的特性[J]. 微生物学通报, 2011, 38(10):1488-1498.
[11] Brown J K M, Wolfe M S. Structure and evolution of a population of Erysiphe graminis f. sp. Hordei[J]. Plant Pathology, 1990, 39(3):376-390.
[12] Yang X J, Yang L J, Yu D Z, et al. Effects of physcion, a natural anthraquinone derivative on the infection process of Blumeria graminis on wheat[J]. Canadian Journal of Plant Pathology, 2008, 30(3):391-396.
[13] Gong S J, Hao J J, Xia Y Y, et al. Inhibitory effect of bionic fungicide 2-allylphenol on Botrytis cinerea (Pers. ex Fr.) in vitro[J]. Pest Management Science, 2009, 65(12):1337-1343.
[14] Buchanan R E, Gibbons N E. Bergey's Manual of Determinative Bacteriology (8th Edition)[M]. Baltimore:The Williams and Wilkins Company Press, 1974, 81-97.
[15] 赵朋超, 王建华, 权春善, 等. 枯草芽孢杆菌抗菌肽生物合成的研究进展[J]. 中国生物工程杂志, 2010, 30(10):108-113.
[16] 唐容容, 杨文革, 胡永红, 等. 蜡样芽孢杆菌CGMCC4348菌株防治番茄灰霉病的效果及机理研究[J]. 湖北农业科学, 2013, 52(8):1817-1820.
[17] 张彦, 车建美, 刘波, 等. 蜡样芽孢杆菌ANTI-8098A在番茄内的定殖和对青枯病的防治研究[J]. 中国生物防治学报, 2011, 27(2):221-227.
[18] 韩艳霞, 胡斌杰, 王宫南. 蜡样芽孢杆菌JK14对小麦全蚀病的防治及其抑病机理[J]. 农业科学与技术, 2008, 9(1):70-74.
[19] 曾凡松, 向礼波, 杨立军, 等. 一株内生细菌EA19的分离鉴定及其对小麦白粉病菌的抑制效果[J]. 湖北农业科学, 2012, 51(23):5344-5347.
[20] 杨振娟, 阿拉坦夫, 孙蕾, 等. 拮抗细菌A2菌株的鉴定及其对小麦白粉病的防治效果[J]. 中国农学通报, 2012, 28(9):185-189.