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journal1 ›› 2016, Vol. 32 ›› Issue (5): 635-641.DOI: 10.16409/j.cnki.2095-039x.2016.05.013

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Antifungal Activity and Mechanism of an Endophytic Bacterium ZY-1 from Wheat Root against Blumerial graminis f. sp. Tritici

GONG Shuangjun, XIANG Libo, XUE Minfeng, ZHANG Xuejiang, SHI Wenqi, ZENG Fansong, WANG Hua, YANG Lijun, YU Dazhao   

  1. Key Laboratory of Integrated Pest Management on Crop in Central China, Ministry of Agriculture/Hubei Province Key Laboratory for Control of Crop Diseases, Pest and Weeds/Institute of Plant Protection and Soil Science, Hubei Academy of Agricultural Sciences, Wuhan 430064, China
  • Received:2016-03-16 Online:2016-10-08 Published:2016-10-08

Abstract: Six Bacillus strains isolated from wheat roots were evaluated for antifungal activity using oil-spreading and inhibitory tests. One strain, ZY-1, was found to have higher activity than the others, and PCR analysis revealed that it contained at least four antifungal genes including srfA, fenA, ituB, and bacD. Phylogenetic analysis of its 16S rDNA gene revealed that ZY-1 was a new strain of Bacillus cereus. Further investigation using in vitro plate assays and greenhouse experiments revealed that strain ZY-1 provided better control on Blumeria graminis f. sp. tritici than 10 mg/L applications of the chemical fungicide triadimefon, with control efficacies of 97.94%±1.25% and 82.56%±2.11%, respectively (P<0.05). In vivo histological analysis of the culture filtrate showed strain ZY-1 had a dramatic effect on the development of B. graminis, inhibiting both the germination of conidia and the formation of haustoria (P<0.05). Taken together, these results indicated that the lipopeptide-producing B. cereus stain ZY-1 has great potential as a biological control agent against B. graminis.

Key words: Bacillus cereus, antifungal activity, mechanism, Blumeria graminis f. sp. Tritici

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