Optimization of Fermentation Conditions of Antagonistic Fungus LMNS-M9 against Quinoa Basal Stem Rot and Development of Wettable Powder

doi:10.16409/j.cnki.2095-039x.2025.02.051

Abstract

Abstract: In order to develop a biocontrol agent for the prevention and control of quinoa basal stem rot, this study utilized Trichoderma afroharzianum LMNS-M9 as the primary research object. Its antifungal activity was determined, and then the single-factor and response surface tests were designed and performed to optimize the fermentation condition with the inhibition rate as the indexes. On this basis, the carriers and additives were screened to make wettable powder, and then the formula was optimized and the quality was tested. The results showed that strain LMNS-M9 possess high and broad-spectrum antifungal activity, and the inhibition rate against Fusarium solani reached 69.59%. The optimal fermentation conditions of strain LMNS-M9 were as follows: liquid volume 118.84 mL/250 mL, inoculum 0.51%, fermentation time 114.24 h, and rotation speed 210 r/min. After optimization, the inhibition rate of the spore solution of strain LMNS-M9 against F. solani was 86.98%, which was 125.00% higher than that before optimization. The composition of LMNS-M9 wettable powder obtained by adjuvant screening and formulation optimization is as follows: spore powder 20%, diatomite 67%, polyethylene glycol 3%, NNO 7%, dextrin 3%, spore content was 3.08×10⁹ CFU/g, all indicators meet the national standards. The results can provide a theoretical basis for the green control of quinoa basal stem rot and the industrial production of LMNS-M9 wettable powder.

Key words: Trichoderma afroharzianum, Fusarium solani, fermentation conditions, wettable powder

CLC Number:

S476

ZHANG Wenjing, XU Zilu, Lü Hong, QIN Nan, YIN Hui, ZHAO Xiaojun, REN Lu. Optimization of Fermentation Conditions of Antagonistic Fungus LMNS-M9 against Quinoa Basal Stem Rot and Development of Wettable Powder[J]. Chinese Journal of Biological Control, 2025, 41(4): 862-876.

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