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Chinese Journal of Biological Control ›› 2024, Vol. 40 ›› Issue (2): 424-434.DOI: 10.16409/j.cnki.2095-039x.2024.02.005

• RESEARCH REPORTS • Previous Articles    

Identification,Fermentation Optimization and Determination of Nematicidal Activity of Biocontrol Bacterium Y26

MEI Fengzhen1, ZHOU Mingchao1, YANG Dan1, LI Xiangfeng5, KONG Lingan3, LIU Jianbin4, SHI Fengyu1,2   

  1. 1. College of Agronomy and Biotechnology, Hebei Normal University of Science and Technology, Changli 066004, China;
    2. Key Laboratory of crop stress biology, Hebei Normal University of Science and technology, Changli 066004, China;
    3. State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    4. Institute of Plant Nutrition, Resources and Environment, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China;
    5. Handan Garden Bureau of Hebei Province, Handan 056000, China
  • Received:2023-09-01 Published:2024-04-28

Abstract: To control the root-knot nematode disease in cucumber,a bacterial strain Y26 was isolated from cucumber rhizosphere soil in the laboratory.Bio-activity of fermentation broth against root-knot nematodes was determined.The corrected mortality rate of the second-stage juveniles treated with fermentation broth for 24 hours was 95.12%,and the relative inhibition rate of eggs hatching reached 83.51% after 48 hours of fermentation broth treatment,so strain Y26 can be used as a bacterial control resource for root-knot nematodes disease.Strain Y26 was identified as Bacillus pumilus by morphological observation and molecular identification.The optimized conditions for fermentation of strain Y26 by Plackett-Burma design and response surface analysis were corn flour 2.14%,bean cake flour 3%,K2HPO4 0.57%,MgSO4·7H2O 0.21%,cultural time 12 hours,pH 7.2,inoculation amount 2%,cultural temperature 28℃,and the media amount was 50 mL/250 mL.After fermentation optimization,the spore concentration was increased by 25.69%,the corrected mortality rate of 10% fermentation broth increased from 78.03% to 93.32% before optimization.The results showed that the activity of optimized Y26 fermentation broth root-knot nematodes was significantly increased and could be developed and applicated as a biological nematicide.

Key words: root-knot nematode, Bacillus pumilus, identification, optimization of fermentation conditions

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