Control Efficacy and Mechanism of Biocontrol Bacterium PHJ10 against Wheat Fusarium Head Blight

doi:10.16409/j.cnki.2095-039x.2026.02.058

Abstract

Abstract: Fusarium head blight, caused by Fusarium species, is a devastating wheat disease that causes substantial yield losses. Meanwhile, mycotoxins such as deoxynivalenol (DON) produced by the pathogen seriously threaten human and livestock health. In this study, a bacterial strain PHJ10 was screened, which exhibited inhibition rate 45.99% against mycelial growth of Fusarium graminearum. Based on morphological, physiological-biochemical characteristics together with sequence analysis of 16S rRNA and gyrB genes, the strain PHJ10 was identified as Bacillus atrophaeus. PHJ10 treatment caused morphological abnormalities of fungal hyphae, exerted significant biocontrol effects on wheat coleoptile-and spike-associated diseases, and reduced the contents of DON and its masked derivative deoxynivalenol-3-glucoside (D3G) in grains by 31.01% and 25.38%, respectively. Transcriptomic analysis revealed that PHJ10 interfered with pathways including ribosome biosynthesis and endoplasmic reticulum protein processing in F. graminearum, and impaired fatty-acid degradation and amino-acid metabolism, thereby exerting multi-target impacts on the translation and metabolic systems of the pathogen. These results demonstrate that Bacillus atrophaeus PHJ10 exhibits favorable biocontrol efficacy against Fusarium head blight and holds promise for biocontrol applications.

Key words: Fusarium graminearum, Bacillus atrophaeus, inhibition rate, deoxynivalenol, biocontrol

CLC Number:

S476

PENG Huijuan, WENG Lina, LIAO Yihua, MA Dongfang, LIU Xin, LI Yan, XING Yujun. Control Efficacy and Mechanism of Biocontrol Bacterium PHJ10 against Wheat Fusarium Head Blight[J]. Chinese Journal of Biological Control, 2026, 42(5): 1242-1253.

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References

[1] Xu M, Wang Q, Wang G, et al. Combatting Fusarium head blight:Advances in molecular interactions between Fusarium graminearum and wheat[J]. Phytopathology Research, 2022, 4(1):37.
[2] 宗保升,杜冬梅,高洁,等.小麦镰刀菌病害及其防治研究进展[J].山东农业科学, 2025, 57(8):168-173.
[3] 康梦倩,刘旬胜,刘华丽.小麦赤霉病防治误区与综合防控策略[J].现代农村科技, 2025(11):59-60.
[4] Altinkaynak H, Ozkoc I. Isolation and molecular characterization of plant growth promoting bacteria from the rhizosphere of orchids in Turkey[J]. Rhizosphere, 2020, 16:100280
[5] Esuola C O, Tovignan K T. Plant growth-promoting rhizobacteria (PGPR) native to African yam bean (Sphenostylis stenocarpa) enhance the early growth of three economically important legume species[J]. Plant Physiology, 2024, 29:514-523.
[6] Aslam M M, Okal E J, Idris A L, et al. Rhizosphere microbiomes can regulate plant drought tolerance[J]. Pedosphere, 2022, 32(1):61-74.
[7] Nguyen T B H, Pensec F, Mounier J, et al. A workflow for sourcing and characterizing wheat field microorganisms for the biocontrol of Fusarium head blight[J]. Biological Control, 2025, 209:105856.
[8] Kgosiemang J L, Ramakuwela T, Figlan S, et al. Antifungal effect of metabolites from bacterial symbionts of entomopathogenic nematodes on Fusarium head blight of wheat[J]. Journal of Fungi, 2024, 10(2):148.
[9] Zubair M, Farzand A, Mumtaz F, et al. Novel genetic dysregulations and oxidative damage in Fusarium graminearum induced by plant defense eliciting psychrophilic Bacillus atrophaeus TS1[J]. International Journal of Molecular Sciences, 2021, 22(22):12094.
[10] 李红,牛新湘,吴凤康,等.萎缩芽孢杆菌抑菌物质的鉴定及其对厚皮甜瓜贮藏期风味品质的影响[J].园艺学报, 2025, 52(7):1901-1914.
[11] 梁玉婷,任兴平,李惠霞,等.生防芽孢杆菌种子处理剂WS20的研制及对甘蓝枯萎病的防治效果[J].农药学学报, 2024, 26(3):482-491.
[12] Bian R, Ren H, Jiang M, et al. Cryphonectria hypovirus 1 infection suppresses the pathogenicity but increases the mycotoxin deoxynivalenol production of Fusarium graminearum[J]. Phytopathology Research, 2024, 6:54.
[13] Hussain S, Ali M, Ghazy A, et al. Identification of antifungal lipopeptides from Bacillus subtilis Sh-17 targeting Fusarium oxysporum f. sp. lycopersici[J]. Chemical and Biological Technologies in Agriculture, 2025, 12:29.
[14] 梁玉婷,石海春,李惠霞,等.萎缩芽孢杆菌ZY1825对5种牧草病害的拮抗效果及优化发酵条件[J].草业科学, 2025, 42(1):90-100.
[15] 刘思煜,黑雅娅,薛应钰.南瓜枯萎病生防细菌YB菌株的鉴定和生防效果[J].中国生物防治学报, 2026, 42(3):695-708.
[16] 郭致杰,徐生军,荆卓琼,等.菌株HMQ20YJ11的筛选鉴定及对辣椒疫病的抑制评价[J].寒旱农业科学, 2022, 1(10):88-93.
[17] 朱杰,程亮,张纲,等.樱桃叶斑病生防菌株萎缩芽孢杆菌菌株QH-588的筛选鉴定[J].南方农业学报, 2021, 52(11):3022-3033.
[18] Xu Z, Lu H, Shi W, et al. Optimization of fermentation and biocontrol efficacy of Bacillus atrophaeus XHG-1-3m2[J]. Microorganisms, 2024,12(11): 2134.
[19] 刘锐,韩龙,何张江,等.小麦赤霉病生防菌的筛选及其活性物的初步研究[J].东北农业科学, 2023, 48(6):90-95, 100.
[20] 李修善.阿式肠杆菌和萎缩芽孢杆菌扫描电镜样品制备观察和分析[J].曲阜师范大学学报(自然科学版), 2016, 42(1):102-104.
[21] Tian Y, Zhang D, Cai P, et al. Elimination of Fusarium mycotoxin deoxynivalenol (DON) via microbial and enzymatic strategies:current status and future perspectives[J]. Trends in Food Science&Technology, 2022, 124:96-107.
[22] Beyari E A. Alternatives to chemical pesticides:the role of microbial biocontrol agents in phytopathogen management:a comprehensive review[J]. Journal of Plant Pathology Microbiology, 2025, 107:291-314.
[23] Ni M, Wu Q, Wang J, et al. Identification and comprehensive evaluation of a novel biocontrol agent Bacillus atrophaeus JZB120050[J]. Journal of Environmental Science and Health Part B, 2018, 53(12):777-785.
[24] Mu Y, Yue Y, Gu G, et al. Identification and characterization of the Bacillus atrophaeus strain J-1 as biological agent of apple ring rot disease[J]. Journal of Plant Diseases and Protection, 2020, 127(3):367-378.
[25] Guardado-Valdivia L, Tovar-Pérez E, Chacón-López A, et al. Identification and characterization of a new Bacillus atrophaeus strain B5 as biocontrol agent of postharvest anthracnose disease in soursop (Annona muricata) and avocado (Persea americana)[J]. Microbiological Research, 2018, 210:26-32.
[26] Wang Y, Ji S, Chen X, et al. Butylated hydroxyanisole controls anthracnose on postharvest citrus fruit by inducing autophagosome formation in Colletotrichum gloeosporioides[J]. Postharvest Biology and Technology, 2024, 210:112740.
[27] Zhang X, Li B, Wang Y, et al. Lipopeptides, a novel protein, and volatile compounds contribute to the antifungal activity of the biocontrol agent Bacillus atrophaeus CAB-1[J]. Applied Microbiology and Biotechnology, 2013, 97(21):9525-9534.
[28] Hao Q, Albaghdady D M D, Xiao Y, et al. Endophytic Metarhizium anisopliae is a potential biocontrol agent against wheat Fusarium head blight caused by Fusarium graminearum[J]. Journal of Plant Pathology, 2021, 103(3):875-885.
[29] Sun L, Chen Y, Liu S, et al. Biocontrol performance of a novel Bacillus velezensis L33a on tomato gray mold and its complete genome sequence analysis[J]. Postharvest Biology and Technology, 2024, 213:112925.
[30] Zakharova A A, Efimova S S, Ostroumova O S. State of the art of cyclic lipopeptide-membrane interactions:pore formation and bilayer permeability[J]. Pharmaceutics, 2025, 17(9):1142.
[31] Hanif A, Zhang F, Li P, et al. Fengycin produced by Bacillus amyloliquefaciens FZB42 inhibits Fusarium graminearum growth and mycotoxins biosynthesis[J]. Toxins, 2019, 11(5):295.
[32] Gu Q, Yang Y, Yuan Q, et al. Bacillomycin D produced by Bacillus amyloliquefaciens is involved in the antagonistic interaction with the plant-pathogenic fungus Fusarium graminearum[J]. Applied and Environmental Microbiology, 2017, 83(19):e01075-17.
[33] Ding Z, Fu W, Zhou P, et al. Wheat-associated antagonistic bacteria Exerts biocontrol activity against fungal growth and deoxynivalenol production in Fusarium graminearum[J]. Current Microbiology, 2025, 83:97.
[34] Kalogeropoulou E, Beris D, Tjamos S E, et al. Arabidopsis β-amylase 3 affects cell wall architecture and resistance against Fusarium oxysporum[J]. Physiological and Molecular Plant Pathology, 2023, 124:101945.
[35] Ge J, Zhai H, Tang L, et al. FgUbiH is essential for vegetative development, Energy metabolism, and antioxidant activity in Fusarium graminearum[J]. Microorganisms, 2024, 12(10):2093.