Effect of Root Exudates of Panax notoginseng under Saponin Stress on Survival of Pseudomonas monteilii PM41 in Soil

doi:10.16409/j.cnki.2095-039x.2026.02.030

Abstract

Abstract: This study aimed to investigate the effects of root exudates of Panax notoginseng under saponin stress and their compounds on the induction, growth, biofilm formation, and soil population dynamics of the beneficial bacterium Pseudomonas monteilii PM41, with the goal of elucidating the recruitment of PM41 by P. notoginseng root exudates and providing a novel strategy for mitigating continuous cropping obstacles in P. notoginseng. The results showed that root exudates of P. notoginseng under saponin stress significantly induced PM41. Further analysis indicated that 4-methoxycinnamic acid and traumatic acid, among the identified compounds, exerted pronounced promoting effects on the induction, and biofilm formation of this strain. Soil survival assays demonstrated that both compounds significantly increased the survival rate of PM41 in sterilized soil, with increases of 77.46% and 101.41%, respectively, outperforming the total root exudate treatment. Collectively, root exudates of P. notoginseng under saponin stress significantly promoted the survival and colonization of PM41, with 4-methoxycinnamic acid and traumatic acid potentially serving as key compounds mediating this process.

Key words: saponin, Panax notoginseng, replant failure, biocontrol bacteria, root exudates, abiotic stress

CLC Number:

S476

ZHONG Shijun, DENG Linmei, LUO Yue, CHEN Lelin, KONG Weiran, YANG Shaolei, ZHU Shusheng, YANG Min, LUO Lifen. Effect of Root Exudates of Panax notoginseng under Saponin Stress on Survival of Pseudomonas monteilii PM41 in Soil[J]. Chinese Journal of Biological Control, 2026, 42(3): 648-659.

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References

[1] 苏豹.三七产业发展及前景分析[J].中国民族民间医药, 2013, 22(13):23-24.
[2] 石礼平,张国壮,刘丛盛,等.三七化学成分和药理作用研究概况及质量标志物的预测[J].中国中药杂志, 2023, 48(8):2059-2067.
[3] Wei W, Yang M, Liu Y X, et al. Fertilizer N application rate impacts plant-soil feedback in a sanqi production system[J]. The Science of the Total Environment, 2018, 633:796-807.
[4] Luo L F, Guo C W, Wang L T, et al. Negative plant-soil feedback driven by re-assemblage of the rhizosphere microbiome with the growth of Panax notoginseng[J]. Frontiers in Microbiology, 2019, 10:1597.
[5] Wang Q J, Ma Y, Yang H, et al. Effect of biofumigation and chemical fumigation on soil microbial community structure and control of pepper Phytophthora blight[J]. World Journal of Microbiology and Biotechnology, 2014, 30(2):507-518.
[6] Yang M, Yuan Y, Huang H C, et al. Steaming combined with biochar application eliminates negative plant-soil feedback for sanqi cultivation[J]. Soil and Tillage Research, 2019, 189:189-198.
[7] Williamson-Benavides B A, Dhingra A. Understanding root rot disease in agricultural crops[J]. Horticulture, 2021, 7(2):33.
[8] Liu H J, Wu J Q, Su Y W, et al. Allyl isothiocyanate in the volatiles of brassica juncea inhibits the growth of root rot pathogens of Panax notoginseng by inducing the accumulation of ROS[J]. Journal of Agricultural and Food Chemistry, 2021, 69:13713-13723.
[9] 王罗涛,杨冬英,邓琳梅,等.三七根际土壤中皂苷类自毒物质降解拮抗细菌的分离筛选[J].南方农业学报, 2020, 51(2):305-312.
[10] 邵秋雨,董醇波,韩燕峰,等.植物根际微生物组的研究进展[J].植物营养与肥料学报, 2021, 27(1):144-152.
[11] Shi Y H, Pan Y S, Xiang L, et al. Assembly of rhizosphere microbial communities in Artemisia annua:recruitment of plant growth-promoting microorganisms and inter-kingdom interactions between bacteria and fungi[J]. Plant and Soil, 2020, 470:127-139.
[12] Hou S J, Thiergart T, Vannier N, et al. A microbiota-root-shoot circuit favours Arabidopsis growth over defence under suboptimal light[J]. Nature Plants,2021, 7(8):1078-1092.
[13] Zambelli A, Nocito F F, Araniti F. Unveiling the multifaceted roles of root exudates:chemical interactions, allelopathy, and agricultural applications[J].Agronomy, 2025, 15(4):845.
[14] Ma H Y, Ren Z T, Luo A H, et al. Self-alleviation of continuous-cropping obstacles in potato via root-exudate-driven recruitment of growth-promoting bacteria[J]. Plant Communications, 2025, 6(7):101372.
[15] Wen T, Yuan J, He X M, et al. Enrichment of beneficial cucumber rhizosphere microbes mediated by organic acid secretion[J]. Horticulture Research,2020, 7:154.
[16] Wankhade A, Wilkinson E, Britt D W, et al. A review of plant-microbe interactions in the rhizosphere and the role of root exudates in microbiome engineering[J]. Applied Sciences, 2025, 15(13):7127.
[17] 刘泽琴,刘宁,李淑娟,等.紫云英与油菜间作模式下根系分泌物对土壤微生物的影响[J].华中农业大学学报, 2023, 42(4):177-184.
[18] 符厚隆,廖道龙,刘子凡,等.南瓜作砧木嫁接冬瓜根系分泌物对土壤微生态的影响[J].中国瓜菜, 2022, 35(6):56-61.
[19] Feng H C, Fu R X, Hou X Q, et al. Chemotaxis of beneficial rhizobacteria to root exudates:the first step towards root-microbe rhizosphere interactions[J].International Journal of Molecular Sciences, 2021, 22(13):6655.
[20] 陈淋,王兴红,刘云鹏.增强贝莱斯芽胞杆菌CLA178根际定殖的蔷薇根系分泌组分鉴定[J].南京农业大学学报, 2021, 44(3):497-505.
[21] Wen T, Ding Z X, Thomashow L S, et al. Deciphering the mechanism of fungal pathogen-induced disease-suppressive soil[J]. New Phytologist, 2023,238(6):2634-2650.
[22] Ma W M, Tang S H, Deng Z, et al. Root exudates contribute to belowground ecosystem hotspots:A review[J]. Frontiers in Microbiology, 2022, 13:937940.
[23] Luo L F, Wang Z P, Yan X B, et al. Diversified alternaria pathogenicity alters plant-soil feedbacks through leaf-root-microbiome dynamics in agroforestry systems[J]. Horticulture Research, 2025, 12(8):uhaf137.
[24] Ma S S, Chen Q R, Zheng Y F, et al. A tale for two roles:Root-secreted methyl ferulate inhibits P. nicotianae and enriches the rhizosphere Bacillus against black shank disease in tobacco[J]. Microbiome, 2025, 13(1):33.
[25] Fang H Y, Guo C W, Mei X Y, et al. Light stress elicits soilborne disease suppression mediated by root-secreted flavonoids in Panax notoginseng[J].Horticulture Research, 2024, 11(10):uhae213.
[26] Liu H J, Su Y W, Ye C, et al. Nucleotides enriched under heat stress recruit beneficial rhizomicrobes to protect plants from heat and root-rot stresses[J].Microbiome, 2025, 13(1):160.
[27] Deng L M, Luo L F, Li Y, et al. Autotoxic ginsenoside stress induces changes in root exudates to recruit the beneficial Burkholderia strain B36 as revealed by transcriptomic and metabolomic approaches[J]. Journal of Agricultural and Food Chemistry, 2023, 71(11):4536-4549.
[28] Luo L F, Yang L, Yan Z X, et al. Ginsenosides in root exudates of Panax notoginseng drive the change of soil microbiota through carbon source different utilization[J]. Plant and Soil, 2020, 455:139-153.
[29] Liu Y P, Feng H C, Fu R X, et al. Induced root-secreted D-galactose functions as a chemoattractant and enhances the biofilm formation of Bacillus velezensis SQR9 in an McpA-dependent manner[J]. Applied Microbiology and Biotechnology, 2020, 104(17):785-797.
[30] 王李俊,杨琴,叶敏,等.苦参总碱抗表皮葡萄球菌的作用机制研究[J].中草药, 2019, 50(24):6032-6037.
[31] Gu Y, Wang X F, Yang T J, et al. Chemical structure predicts the effect of plant-derived low-molecular weight compounds on soil microbiome structure and pathogen suppression[J]. Functional Ecology, 2020, 34(10):2158-2169.
[32] Chen L, Liu Y. The function of root exudates in the root colonization by beneficial soil rhizobacteria[J]. Biology, 2024, 13(2):95.
[33] Sasse J, Martinoia E, Northen T. Feed your friends:Do plant exudates shape the root microbiome?[J]. Trends in Plant Science, 2018, 23(1):25-41.
[34] Huang X F, Chaparro J M, Reardon K F, et al. Rhizosphere interactions:root exudates, microbes, and microbial communities[J]. Botany-botanique, 2014,92(4):281-289.
[35] 张春乐.抑制剂对蘑菇酪氨酸酶的抑制效应及抗菌活性[D].厦门:厦门大学, 2007.
[36] Zhang N, Wang D D, Liu Y P, et al. Effects of different plant root exudates and their organic acid components on chemotaxis, biofilm formation and colonization by beneficial rhizosphere-associated bacterial strains[J]. Plant and Soil, 2014, 374(1):689-700.
[37] 李梦莎,阎秀峰.植物的环境信号分子茉莉酸及其生物学功能[J].生态学报, 2014, 34(23):6779-6788.
[38] Kulkarni O S, Mazumder M, Kini S. et al. Volatile methyl jasmonate from roots triggers host-beneficial soil microbiome biofilms[J]. Nature Chemical Biology, 2024, 20(4):473-483.
[39] Scharf B E, Hynes M F, Alexandre G M. Chemotaxis signaling systems in model beneficial plant-bacteria associations[J]. Plant molecular biology, 2016,90(6):549-559.
[40] Asafova E V, Asaleeva G A, Yakovleva V G, et al. The effect of traumatic acid on tyrosine phosphorylation of proteins in pea seedlings[J]. Doklady Biochemistry and Biophysics, 2005, 405:426-428.
[41] Jabońska-Trypu A, Krętowski R, Wołejko E, et al. Traumatic acid toxicity mechanisms in human breast cancer MCF-7cells[J]. Regulatory Toxicology and Pharmacology, 2019, 106:137-146.
[42] Carvalhais L C, Dennis P G, Badri D V, et al. Linking jasmonic acid signaling, root exudates, and rhizosphere microbiomes[J]. Molecular Plant-Microbe Interactions, 2015, 28(9):1049-1058.
[43] 张钰玲.茉莉酸激素与微生物互作研究进展[J].微生物前沿, 2025, 14(2):59-65.
[44] Li H T, Zhang L, Li J, et al. Resistant starch intake facilitates weight loss in humans by reshaping the gut microbiota[J]. Nature Metabolism, 2024, 6(3):578-597.