Abstract: Metabolism of glycogen and triglyceride in 5 d old adults of Agrypon flexorius exposed to tethered flight under different temperature and humidity conditions were measured. Flight temperature and humidities showed significantly effects on the content of flight energy substances. Less energy substances including triglyceride and glycogen were used when the moths were flied at suitable temperature of 20—24 ℃and humidity of about 55%. Consumption of energy substances during flight also differed between sexes, more consumption was found in female than in male moths during flight at high temperature and extreme high and low humidity.