Impact on Strain Phenotypes and Spinosad Biosynthesis by Disruption of Phosphomannomutase Gene in Saccharopolyspora spinosa

doi:10.16409/j.cnki.2095-039x.2017.01.019

Abstract

Abstract: The phosphomannomutase encoded by manB gene widely exists in eukaryotes, phosphate-6-mannose can be converted into phosphate-1-mannose under the action of phosphomannomutase, which was closely related to primary metabolism and secondary metabolites synthesis process. Previous reports showed that genetic modification on genes related to primary metabolism in Saccharopolyspora spinosa could improve spinosad yields. The 1.0 kb partial segment of manB gene was amplified by PCR and cloned into the vector pOJ260, generating manB disruption vector pOJ260-manB. This vector was introduced into S. spinosa by conjugation, and transconjugant S. spinosa-△manB was obtained successfully. On BHI and TSB agar, the spore germination of the mutant strain S. spinosa-△manB was advanced and the spore yield was more than the wild type strain. HPLC analysis showed that spinosad yield of the mutant strain increased 180% than the wild strain. Our results revealed that disruption of manB affected the development of S. spinosa and the biosynthesis of spinosad.

Key words: Saccharopolyspora spinosa, phosphomannomutase gene, disruption, spinosad

CLC Number:

S476

LIU Hongxue, CAI Mei, ZHANG Youheng, XIAO Jie, DING Xuezhi, SUN Yunjun, XIA Liqiu. Impact on Strain Phenotypes and Spinosad Biosynthesis by Disruption of Phosphomannomutase Gene in Saccharopolyspora spinosa[J]. journal1, 2017, 33(1): 134-141.

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