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Chinese Journal Of Biological Control ›› 2020, Vol. 36 ›› Issue (2): 203-210.DOI: 10.16409/j.cnki.2095-039x.2019.06.011

• RESEARCH REPORTS • Previous Articles     Next Articles

Ligands Binding Characteristics of Odorant Binding Proteins AlucOBP33 of Apolygus lucorum (Hemiptera:Miridae)

SUN Xiaojie1,2, LI Zibo2, ZHANG Qiang2, LIU Yunze1,2, ZHENG Fangqiang1, ZHANG Yongjun2   

  1. 1. College of Plant Protection, Shandong Agricultural University, Tai'an 271018, China;
    2. State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
  • Received:2019-06-11 Online:2020-04-08 Published:2020-04-16

Abstract: To investigate the roles of odorant binding protein AlucOBP33 in chemoreception of Apolygus lucorum, the ligand binding characteristics of AlucOBP33 was studied in laboratory. The candidate AlucOBP33 was obtained from antennal transcriptome data of A. lucorum. The 3D model predicts that AlucOBP33 belongs to classic OBPs which contain six alpha helix and three pairs of cysteine. AlucOBP33 was cloned, prokaryotic expressed and further purified by using affinity chromatography. The fluorescence competitive binding experiment was performed to determine the binding abilities of AlucOBP33 to 73 odorant chemicals and 13 taste compounds. It was found that recombinant AlucOBP33 bound effectively with two alcohols, four ketones, one ester, one aldehyde, one alkene, and four taste compounds. We propose that AlucOBP33 play dual roles in olfactory perception and taste recognition of A. lucorum.

Key words: Apolygus lucorum, odorant binding protein, fluorescence competitive binding assays, chemoreception

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