Visual Detection of Entomogenous Fungi Isaria fumosorosea by Loop-mediated Isothermal Amplification Technology

doi:10.16409/j.cnki.2095-039x.2020.05.015

Abstract

Abstract: Isaria fumosorosea is an important entomogenous fungus and has become an important resource for microbial pesticides. Due to the lack of effective methods, a large number of rapid and accurate detections of I. fumosorosea in the diseased pests are impossible, which limits the scientific evaluation of the control effect of related biocontrol strains. In this study, through the primers designed and system optimization of the loop-mediated isothermal amplification (LAMP), a visual LAMP detection method was established for I. fumosorosea. The detection method had strong specificity, high accuracy, and no cross reaction with other common biocontrol strains and pathogenic bacteria; the detection time was short, and a positive result could be obtained in 60 minutes; the sensitivity was high, which was 100 fold that of conventional PCR detection methods. The establishment of this method provides a new technology for the virulence determination and safety evaluation of I. fumosorosea under field conditions.

Key words: entomogenous fungi, Isaria fumosorosea, LAMP, visual detection

CLC Number:

S476

LIU Xiaofei, CHEN Ruiyuan, ZHENG Yu. Visual Detection of Entomogenous Fungi Isaria fumosorosea by Loop-mediated Isothermal Amplification Technology[J]. Chinese Journal Of Biological Control, 2020, 36(5): 795-801.

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