Effect of Aureobasidium pullulans PA-2 Metabolism of Chenopodium album Leaves

doi:10.16409/j.cnki.2095-039x.2021.02.010

Abstract

Abstract: To clarify the effect of Aureobasidium pullulans PA-2 infection on metabolism of Chenopodium album leaves, the metabolomics method based on liquid chromatography-mass spectrometry (LC-MS) was used to analyze the differential metabolites in C. album leaves infected by the strain PA-2. Through orthogonal partial least squares discriminant analysis (OPLS-DA), a total of 187 metabolites with significant differences (VIP>1 and P<0.05) were screened 24 h after the treatment of PA-2. Among them, 148 were up-regulated and 37 were down-regulated. Adenosine and betaine can be used as the main biomarkers after 24 h. KEGG enrichment pathway analysis indicated that these differential metabolites were mainly enriched in 3 metabolic pathways, including global and overview maps, amino acid metabolism and carbohydrate metabolism. The obvious changes in purine metabolism, biosynthesis of secondary metabolites, amino acid metabolism and carbohydrate metabolism in C. album leaves may participate in the PA-2 infection to C. album. The above results provide a theoretical basis for further research on the biocontrol mechanism of PA-2 infecting C. album.

Key words: Aureobasidium pullulans PA-2, Chenopodium album, metabolomics, differential metabolites, metabolic pathway

CLC Number:

S476

GAO Hanfeng, YANG Ying, CHEN Hongyu, CHENG Liang, GUO Qingyun. Effect of Aureobasidium pullulans PA-2 Metabolism of Chenopodium album Leaves[J]. Chinese Journal of Biological Control, 2021, 37(2): 286-296.

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