Systemic Resistance to Tobacco Mosaic Virus (TMV) Induced by Protein Rhp-PSP of Rhodopseudomonas palustris and Screening of Interaction Protein

doi:10.16409/j.cnki.2095-039x.2021.06.00

Abstract

Abstract: The activity of defense enzymes, content of malondialdehyde (MDA) and expression level of resistance gene were determined in TMV inoculated tobacco plants induced by protein Rhp-PSP systemic resistance to TMV. The results showed that protein Rhp-PSP treatment increased the activities of phenylalanine ammonia-lyase (PAL), peroxidase (POD) and polyphenol oxidase (PPO) in TMV inoculated tobacco leaves, but decreased the content of MDA, and up-regulated the gene expression of PR-1, PR-3, PR-5 and PDF1.2 significantly. The above results indicated that protein Rhp-PSP of Rhodopseudomonas palustris could induce systemic resistance to TMV in tobacco. In order to understand the mechanism of induce systemic resistance, the interaction proteins with protein Rhp-PSP were screened in the tobacco cDNA library using yeast two-hybrid system. The result showed that the GPI anchoring protein, VAN3 binding protein, chlorophyll binding protein A may have interaction with Rhp-PSP.

Key words: Rhodopseudomonas palustris, defense-related enzyme, plant defense-related genes, protein interaction

CLC Number:

S476

ZHOU Hanmei, CHEN Lijie, ZHAI Zhongying, ZHANG Deyong, SU Pin, LIU Yong. Systemic Resistance to Tobacco Mosaic Virus (TMV) Induced by Protein Rhp-PSP of Rhodopseudomonas palustris and Screening of Interaction Protein[J]. Chinese Journal of Biological Control, 2021, 37(5): 1073-1081.

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