Plant System Resistance Triggered by Root-colonizing Bacillus subtilis PTS-394 and Its Control Effect on Tomato Gray Mold

doi:10.16409/j.cnki.2095-039x.2017.02.012

Abstract

Abstract: The majority of plant growth promoting rhizobacteria (PGPR) has ability to reduce a plant's susceptibility to pathogen attack by activating plant defenses. In this study, the defense-related enzyme activity, signal transduction pathways and control efficiency against tomato gray mold were investigated to clarify the mechanism of tomato systemic resistance triggered by Bacillus subtilis PTS-394. After root drenching with PTS-394, the PAL, PPO, POX, LOX activity of the top tomato leaves increased at 24-72 h and peaked at 72 h, then declined at 96 h. Meanwhile, the key gene NPR1 in tomato resistance signaling pathway and defense gene PR-1a in salicylic acid (SA) signaling pathway were expressed significantly at 24-72 h after treatment with PTS-394. Accordingly, PTS-394 is capable of inducing systemic resistance in tomato plants. Infection experiment of Botrytis cinerea on detached tomato leaves indicated that the lesion area treated with PTS-394 was only half of that in the blank control, and the control efficacy against tomato grey mold was about 47.1%. Pot experiments in greenhouse showed that the control efficacy on tomato gray mold was 58.2%. In summary, the application of Bacillus subtilis PTS-394 could trigger tomato systemic disease resistance which plays an important role in the control of tomato gray mold.

Key words: Bacillus subtilis, tomato, defense-related enzyme, signal transduction pathways

CLC Number:

S476

QIAO Junqing, ZHANG Xinning, LIANG Xuejie, LIU Yongfeng, LIU Youzhou. Plant System Resistance Triggered by Root-colonizing Bacillus subtilis PTS-394 and Its Control Effect on Tomato Gray Mold[J]. journal1, 2017, 33(2/3): 219-225.

References

[1] Beneduzi A, Ambrosini A, Passaglia L. M:Plant growth-promoting rhizobacteria (PGPR):Their potential as antagonists and biocontrol agents[J]. Genetics and Molecular Biology, 2012, 35(4):1044-1051.
[2] 陈志谊, 刘荣, 刘永锋. 水稻纹枯病拮抗细菌B-916的选育[J]. 中国生物防治, 2003, 19(1):15-18.
[3] 陈志谊, 刘永锋, 陆凡. 井冈霉素和生防菌Bs-916协同控病作用及增效机理[J]. 植物保护学报, 2003, 30(4):429-434.
[4] Borriss R. Use of plant-associated Bacillus strains as biofertilizers and biocontrol agents[M]//Maheshwari D K ed. Bacteria in Agrobiology:Plant Growth Responses. Heidelberg:Springer, 2011, 41-76.
[5] Lugtenberg B, Kamilova F. Plant-growth-promoting rhizobacteria[J]. Annual Review of Microbiology, 2009, 63:541-556.
[6] 李智强, 戴良英, 刘雄伦, 等. 植物抗病机制与信号传导研究进展[J]. 作物研究, 2011, (5):526-530.
[7] 李波, 王军, 孙思. 植物诱导抗病机制的研究进展[J]. 中国植保导刊, 2013, 33(9):19-24.
[8] Minami E I, Ozeki Y, Matsuoka M,et al. Structure and some characterization of the gene for phenylalanine ammonia-lyase from rice plants[J]. European Journal of Biochemistry, 1989, 185:19-25..
[9] Shri M, Kumar S D, Trivedi P K, et al. Effect of arsenic on growth, oxidative stress, and antioxidant system in rice seedlings[J]. Ecotoxicology and Environmental Safety, 2009, 72(4):1102-1110.
[10] 王曼玲, 胡中立, 周明全, 等. 植物多酚氧化酶的研究进展[J]. 植物学报, 2005, 22(2):215-222.
[11] Akram A, Ongena M, Duby F, et al. Systemic resistance and lipoxygenase-related defence response induced in tomato by Pseudomonas putida, strain BTP1[J]. BMC Plant Biology, 2008, 8(8):113.
[12] Wang S, Wu H, Zhan J, et al. The role of synergistic action and molecular mechanism in the effect of genetically engineered strain Bacillus subtilisOKBHF in enhancing tomato growth and cucumber mosaic virus resistance[J]. BioControl, 2011, 56(1):113-121.
[13] Choudhary D K, Prakash A, Johri B N. Induced systemic resistance (ISR) in plants:mechanism of action[J]. Indian Journal of Microbiology, 2007, 47(4):289-297.
[14] 任鹏举, 谢永丽, 张岩, 等. 枯草芽孢杆菌OKB105产生的surfactin防治烟草花叶病毒病及其机理研究[J]. 中国生物防治学报, 2014, 30(2):216-221.
[15] Ryu C M, Farag M A, Hu C H, et al. Bacterial volatiles promote growth in Arabidopsis[J]. Proceedings of the National Academy of Sciences of the United States of America, 2003, 100(8):4927-4932.
[16] 刘邮洲, 陈志谊, 梁雪杰, 等. 番茄枯萎病和青枯病拮抗细菌的筛选、评价与鉴定[J]. 中国生物防治学报, 2012, 28(1):101-108.
[17] 江昌俊, 余有本. 苯丙氨酸解氨酶的研究进展[J]. 安徽农业大学学报, 2001, 28(4):425-430.
[18] 朱广廉. 植物生理学实验[M]. 北京:北京大学出版社, 1990, 51-53.
[19] 何思莲, 刘慧霞, 周少基, 等. 甘蔗多酚氧化酶酶学特性及应用研究[J]. 广西大学学报(自然科学版), 2009, 34(6):827-831.
[20] 钟芳, 王璋, 许时婴. 3种脂肪氧合酶酶活测定方法[J]. 无锡轻工大学学报, 2001, 20(1):77-80.
[21] Ferrie B J, Beaudoin N, Burkhart W, et al. The cloning of two tomato lipoxygenase genes and their differential expression during fruit ripening[J]. Plant Physiology, 1994, 106(1):109-118.
[22] Chen G, Hackett R, Walker D, et al. Identification of a specific isoform of tomato lipoxygenase (TomloxC) involved in the generation of fatty acid-derived flavor compounds[J]. Plant Physiology, 2004, 136(1):2641-2651.
[23] Mariutto M, Duby F, Adam A, et al. The elicitation of a systemic resistance byPseudomonas putidaBTP1 in tomato involves the stimulation of two lipoxygenase isoforms[J]. BMC Plant Biology, 2011, 11(2):29.
[24] Tan S, Dong Y, Liao H, et al. Antagonistic bacteriumBacillus amyloliquefaciens, induces resistance and controls the bacterial wilt of tomato[J]. Pest Management Science, 2013, 69(11):1245-1252.
[25] Zhang R S, Liu Y F, Luo C P, et al. Bacillus amyloliquefaciens Lx-11, a potential biocontrol agent against rice bacterial leaf streak[J]. Journal of Plant Pathology, 2012, 94(3):609-619.
[26] Ongena M, Jourdan E, Adam A,et al. Surfactin and fengycin lipopeptides of Bacillus subtilis as elicitors of induced systemic resistance in plants[J]. Environmental Microbiology, 2007, 9(4):1084-1090.
[27] Qiao J, Liu Y, Liang X, et al. Draft genome sequence of root-colonizing bacterium Bacillussp. strain PTS-394[J]. Genome Announcements, 2014, 2(1):e0003814.