Increase of Defense Enzyme Activity and Up-expression of Defense Gene in Tomato Induced by Bacillus amyloliquefaciens B1619

doi:10.16409/j.cnki.2095-039x.2017.02.014

Abstract

Abstract: Bacillus amyloliquefaciens B1619 could suppress Fusarium wilt of tomato. To explore the potential mechanism underlying the induced resistance mediated by B1619, the expression profile of several plant defense-related enzymes, peroxidase (POD), superoxide dismutase (SOD), phenylalanine ammonia-lyase (PAL) and secondary metabolite malondialdehyde (MDA), several enzymes synthesis-related genes, SOD,POD1 and defense-related genePR-1a, were monitored in different groups treated with B1619 or Fusarium oxysporum. Levels of SOD, POD, PAL activities and SOD,POD1,PR-1a were all significantly higher than those of the control, while the level of MDA was significantly lower. The expression level of PR-1a, POD1 and SOD were significantly enhanced after root drench with B1619 fermentation broth. The above results suggested that the induced resistance in tomato by B1619 might be one of the mechanisms to control tomato Fusarium wilt caused by F. oxysporum.

Key words: Bacillus amyloliquefaciens, induced resistance, plant defense-related enzymes, plant defense-related genes

CLC Number:

S476

WANG Luyao, JIANG Panpan, GAN Ying, CHEN Zhiyi, LIU Yongfeng. Increase of Defense Enzyme Activity and Up-expression of Defense Gene in Tomato Induced by Bacillus amyloliquefaciens B1619[J]. journal1, 2017, 33(2/3): 234-240.

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