Screening of Chemically Defined Medium for Antifungal Substance HSAF

doi:10.16409/j.cnki.2095-039x.2022.02.023

Abstract

Abstract: Heat stable antifungal factor (HSAF) is an antifungal active substance produced by Lysobacter enzymogenes OH11, and it can be developed into an environment-friendly bio-pesticide. In order to further improve the HSAF production, the chemically defined medium was acquired through single factor test, and orthogonal test. Its specific components were:glucose 8 g/L, (NH₄) ₂SO₄ 1 g/L, FeCl₃ 8 mg/L, K₂HPO₄1 g/L and KH₂PO₄0.5 g/L, in which the HSAF production reached (215.46±6.85) mg/L. The advantage of this medium is that the components are known and stable, avoiding the fluctuation of fermentation yield caused by complex components in the medium. Moreover, it is conducive to the study of the characteristics of intracellular metabolism and the key affecting factors of HSAF synthesis, so as to improve the yield and reduce the production cost.

Key words: Lysobacter enzymogenes, heat stable antifungal factor, chemically defined medium, biological fungicide

CLC Number:

S476

TANG Bao, WANG Jinzi, ZHAO Yancun, LIU Fengquan. Screening of Chemically Defined Medium for Antifungal Substance HSAF[J]. Chinese Journal of Biological Control, 2022, 38(3): 645-652.

References

[1] 田囡, 王超, 刘新利. 热稳定抗真菌因子HSAF及其生物防治研究进展[J]. 山东轻工业学院学报(自然科学版), 2013, 27(4):1-6.
[2] Li Y Y, Wang H X, Liu Y, et al. Biosynthesis of the polycyclic system in the antifungal HSAF and analogues from Lysobacter enzymogenes[J]. Angewandte Chemie International Edition, 2018, 57(21):6221-6225.
[3] Li S J, Du L C, Yuen G, et al. Distinct ceramide synthases regulate polarized growth in the filamentous fungus Aspergillus nidulans D[J]. Molecular Biology of the Cell, 2006, 17(3):1218-1227.
[4] Lou L L, Qian G L, Xie Y X, et al. Biosynthesis of HSAF, a tetramic acid-containing macrolactam from Lysobacter enzymogenes[J]. Journal of the American Chemical Society, 2011, 133(4):643-645.
[5] Xu L, Wu P, Wright S J, et al. Bioactive polycyclic tetramate macrolactams from Lysobacter enzymogenes and their absolute configurations by theoretical ECD calculations[J]. Journal of Natural Products, 2015, 78(8):1841-1847.
[6] Tang B, Sun C, Zhao Y C, et al. Efficient production of heat-stable antifungal factor through integrating statistical optimization with a two-stage temperature control strategy in Lysobacter enzymogenes OH11[J]. BMC Biotechnology, 2018, 18(1):69.
[7] Li K H, Hou R X, Xu H Y, et al. Two functional acyl-CoA ligases affect free fatty acid metabolism to block biosynthesis of an antifungal antibiotic in Lysobacter enzymogenes[J]. Applied and Environmental Microbiology, 2020, 86(10):e00309-20.
[8] Tang B, Zhao Y C, Shi X M, et al. Enhanced heat stable antifungal factor production by Lysobacter enzymogenes OH11 with cheap feedstocks:medium optimization and quantitative determination[J]. Letters in Applied Microbiology, 2018, 66(5):439-446.
[9] 姜英华. 一种新型生防菌菌株OH11的鉴定和生防效果研究[D]. 南京:南京农业大学, 2006.
[10] 佘永红, 罗瑞明, 刘晓连, 等. 透明质酸发酵过程中底物抑制及解除抑制的方法研究[J]. 安徽农业科学, 2011, 39(17):10104-10107.
[11] 王儒富, 刘勇. 氯化亚铁氧化计量生产高浓度聚氯化铁多元盐净水剂[J]. 四川化工, 2021, 24(3):38-42.
[12] Hallenbeck P C, Grogger M, Mraz M, et al. The use of design of experiments and response surface methodology to optimize biomass and lipid production by the oleaginous marine green alga, Nannochloropsis gaditana in response to light intensity, inoculum size and CO₂[J]. Bioresource Technology, 2015, 184:161-168.
[13] 陈腾飞. 全合成培养基考察木糖等前体物质对庆大霉素C1a产量的影响[D]. 上海:华东理工大学, 2020.
[14] 廖建国, 洪铭, 储炬. 运用高通量筛选技术优化红霉素A发酵的合成培养基[J]. 中国抗生素杂志, 2018, 43(1):51-58.
[15] 王龙, 赵宏图, 于岚, 等. 适合龟裂链霉菌¹³C代谢通量分析合成培养基的优化及应用[J]. 生物工程学报, 2014,30(4):679-683.
[16] 田云龙, 蒋细良, 姬军红, 等. 中生菌素产生菌发酵合成培养基的设计优化[J]. 中国抗生素杂志, 2010, 35(3):189-193.
[17] 谢秋萍, 卫腾云, 杨松柏, 等. 喷他霉素的发酵培养基及工艺研究[J]. 中国医药工业杂志, 2021, 52(4):507-512.
[18] Jiang Y X, Tang B, Xu Z Q, et al.Improvement of poly-γ-glutamic acid biosynthesis in a moving bed biofilm reactor by Bacillus subtilis NX-2[J]. Bioresource Technology, 2016, 218:360-366.
[19] 陆原, 李桢林, 王永红, 等. 铵离子对必特螺旋霉素组分生物合成的调控作用[J]. 微生物学报, 2006(6):928-933.
[20] 张松, 扶教龙, 徐敏强, 等. 铵离子对多杀菌素发酵生产的影响[J]. 苏州科技大学学报(自然科学版), 2021, 38(1):64-70.
[21] 魏永昌, 袁凯凯, 李振海, 等. 出芽短梗霉产黑色素发酵工艺优化[J]. 食品研究与开发, 2021, 42(3):170-176.
[22] De Serrano L O, Camper A K, Richards A M. An overview of siderophores for iron acquisition in microorganisms living in the extreme[J]. Biometals:an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine, 2016, 29(4):551-571.
[23] 李爱江, 张敏, 辛莉. 发酵生产过程中发酵条件对微生物生长的影响[J]. 农技服务, 2007(4):124-126.
[24] 田康明, 尔昊, 路福平, 等. 磷酸盐精确控制策略提升大肠杆菌生长和产物合成效率[J]. 食品工业科技, 2016, 37(1):184-189.
[25] 张晓云, 曲远航, 郭庆港, 等. 萎缩芽胞杆菌HMB22922发酵工艺及其制剂研制[J]. 中国生物防治学报, 2019, 35(5):768-776.