Control Effect of Several Biological Fungicides and Their Mixtures on the Bulb Rot of Lanzhou Lily During Storage

doi:10.16409/j.cnki.2095-039x.2021.06.002

Abstract

Abstract: In order to screen out biological fungicides with high-efficiency and low-toxicity against Lanzhou lily bulb rot disease and alleviate the resistance of pathogen to pesticides in production, inhibition rate, activity and co-toxicity coefficient of several biological fungicides and their mixtures to Fusarim oxysporum were determined by the growth rate method. The results showed that the inhibition rates of the six biological fungicides against F. oxysporum were all above 69%, and the values of EC₅₀in descending order was Paenibacillus polymyxa, Trichoderma viride, phenazine-1-carboxylic acid, Trichoderma harzianum, eugenol and carvacrol. Four kinds of mixtures agents with synergistic effects were obtained, namely paenibacillus polymyxa with eugenol (9:1), paenibacillus polymyxa with eugenol (8:2), paenibacillus polymyxa with chlorothalonil (8:2), and eugenol with chlorothalonil (4:6), and their co-toxicity coefficients were 347.88, 129.73, 440.60 and 641.66, respectively. Compared with the single agent, control effects of these four kinds of mixtures to Lanzhou lily bulb rot disease increased by 5.88%-57.89%. The protective effects of mixture and single agents were higher than the treatment effects.

Key words: lily, Fusarium oxysporum, biological fungicide, virulence, synergistic effect, control

CLC Number:

S476

Jiang Yuling, Liang Qiaolan, Wei Liexin, Meng Xiupeng, Lin Ke, Yue Yang. Control Effect of Several Biological Fungicides and Their Mixtures on the Bulb Rot of Lanzhou Lily During Storage[J]. Chinese Journal of Biological Control, 2021, 37(5): 1041-1049.

References

[1] 李雪, 赵伟, 梁旭东. 实施品牌战略, 促进兰州百合跨越式发展[J]. 农业经济, 2015, 5:20-21.
[2] 刘华, 祁复绒, 周亚婷. 甘肃省百合产业发展调研与思考[J]. 农业科技与信息, 2018, 547(14):40-42.
[3] 徐岩. 兰州百合引种及其栽培措施对生长特性和品质的影响[D]. 长春:吉林大学, 2013.
[4] 黄钰芳, 张恩和, 张新慧, 等. 不同连作年限兰州百合光合特性日变化与生理生态因子的关系[J]. 西北农林科技大学学报(自然科学版), 2020. 10(8):1-9.
[5] 黄钰芳, 张恩和, 张新慧, 等. 兰州百合连作障碍效应及机制研究[J]. 草业学报, 2018, 27(2):146-155.
[6] 任胜林, 梅慧玲, 李映萱, 等.百合枯萎病病原菌的分离鉴定及其接种浓度对发病程度的影响[J]. 南京农业大学学报, 2020, 43(6):1097-1106.
[7] 施祖国. 植物镰刀菌枯萎病防治的研究进展[J]. 现代农业科技, 2016, 20:102-103.
[8] 李诚, 李俊杰. 百合枯萎病研究[J]. 植物病理学报, 1992, 3:26-28.
[9] 朱茂山, 关天舒, 吕振环. 13种杀菌剂对百合枯萎病菌的室内毒力测定[J]. 农药, 2010, 49(10):760-761.
[10] 朱海燕. 龙牙百合鳞茎腐烂病病原鉴定及室内药剂毒力测定[D]. 长沙:湖南农业大学, 2012.
[11] 梁巧兰, 徐秉良, 刘艳梅. 观赏百合根腐病病原鉴定及药剂筛选[J]. 甘肃农业大学学报, 2004, 39(1):25-28.
[12] 叶世森, 林芳. 百合真菌性病害化学防治方法的研究[J]. 福建林业科技, 2007, 3(34):65-68.
[13] 李润根, 王愉, 程华. 百合枯萎病病原菌分离鉴定及药剂室内筛选[J]. 湖北农业科学, 2016, 55(10):2551-2554.
[14] 魏志刚. 东方百合茎腐病病原研究及抗性分析[D]. 南昌:江西农业大学, 2014.
[15] 周佳民, 宋荣, 张天术, 等. 不同土壤消毒剂对药用百合病害防治效果的研究[J]. 山地农业生物学报, 2016, 35(6):76-80.
[16] 梁巧兰, 徐秉良, 刘艳梅. 观赏百合根腐病病原鉴定及药剂筛选[J]. 甘肃农业大学学报, 2004, 39(1):25-28.
[17] 田丽丽, 梁巧兰, 徐秉良, 等. 切花百合枯萎病菌室内药剂筛选及田间防治效果[J]. 甘肃农业大学学报, 2008, 45(5):113-116.
[18] Fravel D R, Olivain C, Alabouvette C. Fusarium oxysporum and its biocontrol[J]. New Phytologist, 2010, 157(3):493-502.
[19] Hua C P. Effects of intercropping on rhizosphere soil microorganisms and root exudates of Lanzhou lily (Lilium davidii var. unicolor)[J]. Sciences in Cold and Arid Regions, 2018, 10(2):159-168.
[20] 黄超杰. 兰州百合根系内生真菌生态学的研究[D]. 兰州:兰州大学, 2010:5.
[21] 刘兆娜, 郭绍霞, 李伟. AM真菌对百合生长和生理特性的影响[J]. 草业学报, 2017, 26(11):85-93.
[22] 喻国辉, 程萍, 王燕鹂, 等. 一株香蕉枯萎病生防芽孢杆菌的鉴定、生物学特性和抗菌谱研究[J]. 中国农学通报, 2010, 26(12):216-220.
[23] 韩玲. 拮抗菌和大蒜对百合枯萎病的抑菌和防病作用研究[D]. 杨凌:西北农林科技大学, 2010, 40.
[24] 武志江, 李业燕, 王亚军, 等. 百合枯萎病拮抗细菌的筛选、鉴定及其抑菌物质研究[J]. 微生物学通报, 2015, 42(7):1307-1320.
[25] Tang W H, Cook R J, Rovira A. Advances in biological control of plant disease[M]. Beijing:China Agricultural University Press, 1996. 327-329.
[26] Alizadeh A, Samih M A. Compatibility of Verticillium lecani (Zimm.) with several pesticides[J]. Communications in Agricultural and Applied Biological Sciences, 2007, 72(4):1011-1015.
[27] 农药室内生物测定试验准则杀菌剂第2部分:抑制病原真菌菌丝生长试验平皿法[M]. 北京:中国农业出版社, 2006, NYT1156.
[28] Horsfall J G. Fungicides and their action[M]. Waltham:Chronica Botanica, 1945.
[29] 王小艺, 王跃龙, 欧晓明. 农药混剂配比研究的一种实用寻优方法初探[J]. 农药学学报, 2005, 7(1):40-44.
[30] 叶正荷, 张功普. 农药混剂中单剂搭配和最佳配比选择研究[J]. 安徽农业科学, 1997, 25(3):246-274.
[31] Mansour N A, Eldefrawi M E, Toppozada A, et al. Toxicological studies on the Egyptian cotton leaf worm, Prodenia litura. vi. potentiation and antagonism of organophosphorus and carbamate insecticides[J]. Journal of Economic Entomology, 1966, 59(2):307-311.
[32] Sun Y P, John E R. Analysis of joint action of Insecticides against house fly[J]. Journal of Economic Entomology, 1960, 53(5):887-892.
[33] Kloepper J W, Leong J, Teintze M, et al. Enhance plant growth by siderophores produced by plant growth-promoting rhizobacteria[J]. Nature, 1980, 286(28):885-886.
[34] Weller D M. Biological control of soilborne plant pathogens in the rhizosphere with bacteria[J]. Annual Review of Phytopathology, 1988, 26(1):379-407.
[35] 梁巧兰, 张娜, 魏列新, 等. 深绿木霉蛋白质TraT2A诱导兰州百合抗灰霉病的作用[J]. 中国生物防治学报, 2017, 33(4):545-551.
[36] 陈长卿, 闫东, 姜云, 等. 生防细菌NJ13与化学农药复配对人参镰刀菌根腐病菌的抑制作用[J]. 中国中药杂志, 2019, 44(10):2015-2019.
[37] 刘磊, 梁昌聪, 杨腊英, 等. 生防芽胞杆菌与化学杀菌剂混配对香蕉枯萎病菌室内毒力测定[J]. 中国南方果树, 2013, 42(6):66-68.
[38] 刘淑娟, 陈秀蓉, 袁宏波, 等. 芽胞杆菌(Bacillus spp.)与甲基硫菌灵混配对茄腐镰孢菌(Fusarium solani)的抑制作用[J]. 植物保护, 2008, 34(5):149-152.
[39] 吉沐祥, 姚克兵, 李国平, 等. 枯草芽胞杆菌DJ-6与吡唑醚菌酯及其混配对草莓枯萎病的室内抑菌活性与田间促生防病效果[J]. 农业科学与技术, 2014, 15(11):2020-2025.
[40] 陈志谊, 任海英, 刘永锋, 等. 戊唑醇和枯草芽胞杆菌协同作用防治蚕豆枯萎病及增效机理初探[J]. 农药学学报, 2002, 4(4):40-44.
[41] Hall R A. Laboratory studies on the effects of fungicides, acaricides and insecticides on the entomopathogenic fungus, Verticillium lecanii[J]. Entomologia Experimentalis et Applicata, 1981, 29(1):39-48.
[42] 柳春燕, 郭敏, 林学政, 等. 拟康氏木霉和枯草芽胞杆菌对黄瓜枯萎病的协同防治作用[J]. 中国生物防治, 2005, 21(3):206-208.
[43] Hammad A M M, EI-Mohandes M AO. Controlling fusarium wilt disease of cucumber plants via antagonistic microorganisms in free and immobilized states[J]. Microbial Research, 1999, 154(2):113-117.
[44] 纪明山, 王英姿, 程根武, 等. 西瓜枯萎病拮抗菌株筛选及田间防效试验[J]. 中国生物防治, 2002, 18(2):71-74.
[45] Duijff B J, Recorbet G, Bakker P A, et al. Microbial antagonism at the root level is involved in the suppression of Fusarium wilt by the combination of nonpathogenic Fusarium oxysporum Fo47 and Pseudomonas putida WCS358[J]. Phytopathology, 1999, 89(11):1073-1079.