Effect of Quorum Sensing Signaling Molecule 3-oxo-C8-HSL on Intestinal Symbiont Microbial Diversity of Nilaparvata lugens Stål

doi:10.16409/j.cnki.2095-039x.2024.07.009

Abstract

Abstract: Bacterial quorum sensing (QS) mediated by acyl homoserine lactone (AHL) molecules is a widespread regulatory system in Gram-negative bacteria. However, the interactions and intestinal immunity between QS and insect gut microbes have not been clarified. In this experiment, we explored the effects of QS on the diversity of insect gut microbial communities and intestinal immunity by feeding a bacterial community sensing signaling molecule, 3-oxooctanoyl-homoserine lactone (3-oxo-C8-HSL), to the Nilaparvata lugens Stål. The results showed that the mortality of N. lugens on the fifth day after 3-oxo-C8-HSL treatment was 61.39% higher than that of the control group, and the survival time was shortened by 5 days. The number of microbial phyla, genera and species (ASV) in the intestinal microorganisms of N. lugens were reduced compared with that of the control group, and the diversity of bacteria was increased and the diversity of fungi was decreased; the relative abundance of the dominant genera in the bacterial and fungal communities was reduced. At the same time, the treatment activated intestinal immunity and upregulated expression of immune effectors. In summary, 3-oxo-C8-HSL had effective insecticidal activity, altered the diversity of N. lugens gut microbial communities and activated the gut immune response of N. lugens.

Key words: Nilaparvata lugens, bacterial quorum sensing, intestinal symbiotic microbes, immune genes, microbial diversity

CLC Number:

S476.1

FAN Linlin, SONG Yang, SHENTU Xuping, YU Xiaoping. Effect of Quorum Sensing Signaling Molecule 3-oxo-C8-HSL on Intestinal Symbiont Microbial Diversity of Nilaparvata lugens Stål[J]. Chinese Journal of Biological Control, 2025, 41(3): 710-720.

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