Regulation of Global Regulator ccpA on Biosynthesis of Ibremycin in Brevibacillus brevis X23

doi:10.16409/j.cnki.2095-039x.2025.02.036

Abstract

Abstract: The catabolite control protein A (CcpA) is involved in the coordination of carbon metabolism and is one of the key regulators of Gram-positive bacteria. Although studies have revealed the important role of CcpA in carbon metabolism, its role in regulating edeine biosynthesis is rarely reported. The synthesis of edeine requires a large amount of energy and precursor substances, and carbon source is the key to providing energy and carbon skeleton, and its metabolism is regulated by CcpA. In this study, the ccpA in wild-type Brevibacillus brevis X23 (X23-WT) strain was knocked out by Red/ET homologous recombination method, and the mutant strain X23ΔccpA was obtained. By pair-culturing assay, qRT-PCR and HPLC-MS quantitative analysis, it was found that the inhibition zone diameter of X23ΔccpA was significantly bigger than that of X23-WT, the expression of edeP in edeine biosynthetic gene cluster was significantly up-regulated, and the peak area of edeine was increased by 44.87%, indicating that CcpA is a negative transcriptional regulator of edeine biosynthesis. This study provides candidate transcriptional regulatory factor elements for the metabolic engineering of edeine.

Key words: edeine, metabolite control protein A, Brevibacillus brevis X23, carbon metabolism

CLC Number:

S476

CHEN Ziyue, FU Jing, ZHANG Liang, LAN Ruoyi, LIU Tianbo, LIU Qingshu, WANG Yunsheng, CHEN Wu. Regulation of Global Regulator ccpA on Biosynthesis of Ibremycin in Brevibacillus brevis X23[J]. Chinese Journal of Biological Control, 2025, 41(4): 826-835.

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