Effects of Two-Component System LiaRS on Biosynthesis of Edeine from Brevibacillus brevis X23

doi:10.16409/j.cnki.2095-039x.2024.02.056

Chinese Journal of Biological Control ›› 2024, Vol. 40 ›› Issue (5): 1036-1044.DOI: 10.16409/j.cnki.2095-039x.2024.02.056

• RESEARCH REPORTS • Previous Articles

Effects of Two-Component System LiaRS on Biosynthesis of Edeine from Brevibacillus brevis X23

QIU Liting¹, ZHANG Liang², LIU Tianbo³, CHAO Jin⁴, CAI Hailin⁵, YI Ke⁶, LIU Qingshu⁷, CHEN Wu¹

1. College of Plant Protection, Hunan Agricultural University, Changsha 410128, China;
2. College of Agronomy, Hunan Agricultural University, Changsha 410128, China;
3. Hunan Tobacco Science Research Institute, Changsha 410004, China;
4. Hunan Xiangxi autonomous prefecture tobacco company technology center, Xiangxi 416000, China;
5. Hunan Provincial Tobacco Company Technology Center, Changsha 410019, China;
6. Hunan Tobacco Industry Co., Ltd. Raw Material Procurement Center, Changsha 410007, China;
7. Hunan Provincial Microbiology Research Institute, Changsha 410009, China

Received:2024-04-01 Published:2024-10-11

Abstract

Abstract: Two-component systems (TCS) perform a regulatory function in the biosynthesis of various secondary metabolites in Bacillus. However, the TCS controlling edeine biosynthesis have seldom been reported. This research, employing bioinformatics analysis, has uncovered the potential regulatory role of the two-component factor LiaRS in the biosynthesis of edeine. By employing the Red/ET homologous recombination technique, the liaS and liaR genes of the wild-type X23 were effectively eliminated, leading to the development of mutant strains. The results from plate culture, qRT-PCR quantitative analysis, and HPLC-MS targeted metabolic profiling demonstrated a significant increase in the diameter of the inhibitory zone for the mutant strains and a notable up-regulation of the expression of the edeine biosynthesis gene clusters. Moreover, the peak area of edeine was found to be 1.3 times that of the wild type. The findings of this research indicate that LiaRS functions as a suppressor of edeine biosynthesis, thereby offering potential gene elements for the metabolic engineering of edeine.

Key words: Brevibacillus brevis, edeine, two-component system, LiaRS, Red/ET recombination

CLC Number:

S476

QIU Liting, ZHANG Liang, LIU Tianbo, CHAO Jin, CAI Hailin, YI Ke, LIU Qingshu, CHEN Wu. Effects of Two-Component System LiaRS on Biosynthesis of Edeine from Brevibacillus brevis X23[J]. Chinese Journal of Biological Control, 2024, 40(5): 1036-1044.

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Effects of Two-Component System LiaRS on Biosynthesis of Edeine from Brevibacillus brevis X23

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