Screening, Identification and Fermentation Condition Optimization of Bacillus amyloliquefaciens CY3 and Its Control Effects on Fusarium Root Rot of Tobacco

doi:10.16409/j.cnki.2095-039x.2025.02.040

Abstract

Abstract: Tobacco is one of the most important economic crops in China. However, tobacco root rot caused by Fusarium spp. has seriously impacted the yield and quality of tobacco. To exploit effective antagonists against tobacco Fusarium root rot, a total of 86 microbial strains were isolated from the collected rhizosphere soil samples via confrontation culture method, of which a bacterial strain CY3 displayed a considerable antagonistic effect against F. oxysporum with an inhibitory rate of 72.6%. Based on morphological, biochemical characteristics and phylogenetic analysis of 16S rRNA and gyrB, the strain CY3 was identified as Bacillus amyloliquefaciens. According to the single factor experiment, the optimal fermentation condition was optimized as followed: YSP cultural medium in an initial pH value of 7, incubation with 220 r/min, at 32 ℃ for 60 h, and then the amount of bacteria was increased to 1.46×10⁹ cfu/mL with an inhibitory rate of 92.3%. Then, the treatment of CY3 fermentation broth with 1×10⁶ cfu/mL concentration, the indoor pot and field control efficacies against Fusarium root rot were 81.1% and 84.8%, respectively, which were higher than that of tebuconazole, difenoconazole and thiophanate methyl. To sum up, the B. altitudinis strain CY3 has potential as a promising biocontrol agent with excellent application prospect, which provided biological control resources of tobacco Fusarium root rot.

Key words: tobacco, Fusarium root rot, Bacillus amyloliquefaciens, biological control

CLC Number:

S476

SUN Song, HAN Huimin, GAO Yuping, LIU Chunju, LIU Tao, TAI Jin, WANG Weiyu, WANG Chuan, FAN Zengbo, ZHANG Xiaoyang, HAN Chao. Screening, Identification and Fermentation Condition Optimization of Bacillus amyloliquefaciens CY3 and Its Control Effects on Fusarium Root Rot of Tobacco[J]. Chinese Journal of Biological Control, 2025, 41(4): 916-926.

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