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中国生物防治学报 ›› 2016, Vol. 32 ›› Issue (2): 135-141.DOI: 10.16409/j.cnki.2095-039x.2016.02.001

• 研究论文 •    下一篇

绿盲蝽对七种锦葵科植物挥发物的EAG和趋向行为反应

张俊宇1,2, 林克剑2, 黄欣蒸2, 耿亭3, 魏洪义1, 张永军2   

  1. 1. 江西农业大学农学院, 南昌 330045;
    2. 中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室, 北京 100193;
    3. 中国农业科学院廊坊科研中试基地, 廊坊 065000
  • 收稿日期:2015-05-21 出版日期:2016-04-08 发布日期:2016-04-08
  • 通讯作者: 魏洪义, 张永军
  • 作者简介:张俊宇,E-mail:zhangjunyu90@126.com
  • 基金资助:
    国家"973"计划(2012CB114104);国家自然科学基金(31272048、31471778)

EAG Responses and Taxis Selection of Green Plant Bug Apolygus lucorum to Volatiles from Seven Species of Kinds of Malvaceae Plants

ZHANG Junyu1,2, LIN Kejian2, HUANG Xinzheng2, GENG Ting3, WEI Hongyi1, ZHANG Yongjun2   

  1. 1. College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China;
    2. State Key Laboratory of Biology for Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    3. Langfang Scientific Research Trial Station, Chinese Academy of Agricultural Sciences, Langfang 065000, China
  • Received:2015-05-21 Online:2016-04-08 Published:2016-04-08

摘要: 研究绿盲蝽Apolygus lucorum(Meyer-Dür)对锦葵科植物挥发物的EAG和趋向行为反应,为研发绿盲蝽引诱剂或驱避剂策略提供科学依据。田间采用动态顶空采样方法收集了7种锦葵科植物的挥发物,通过室内气相-质谱联用系统(GC-MS)进行物质鉴定及定量分析,最终测定了雌、雄绿盲蝽成虫对11种候选特征挥发物的触角电位(EAG)反应和嗅觉趋向选择行为。结果表明,7种锦葵科植物的挥发物种类和含量有较大差异,而且4种棉花品种之间的挥发物差异也很显著。棉花石远321的挥发物总量最大,其中4,8-二甲基-1,3,7-壬三烯(DMNT)含量最高,壬醛和罗勒烯其次。蜀葵的挥发物种类和含量均最少。11种候选特征挥发物均能够引起绿盲蝽雌、雄成虫EAG反应,且雄性成虫的反应值大都高于雌性成虫。其中,绿盲蝽雌、雄成虫触角对反-2-己烯酸丁酯EAG反应值最大,芳樟醇、乙酸叶醇酯、甲基庚烯酮及DMNT也能够激发绿盲蝽较大的EAG反应。在趋向行为检测中发现,DMNT及柠檬烯等对雄性成虫具有吸引作用。对雌性成虫有吸引作用的物质有γ-萜品烯、DMNT、月桂烯、α-石竹烯、罗勒烯及乙酸叶醇酯等,其中γ-萜品烯和乙酸叶醇酯对雌性成虫具有极显著吸引效果。锦葵科候选植物挥发物对绿盲蝽雌、雄虫具有不同EAG和趋向行为反应影响,候选气味化合物可以作为设计绿盲蝽引诱剂组分进行害虫防控田间实践。

关键词: 绿盲蝽, 锦葵科植物, 挥发物, EAG反应, 趋向行为

Abstract: To design the attractants or repellents of the green plant bugs Apolygus lucorum for use in IPM, we investigated the EAG responses and taxis selection of A. lucorum to volatiles from seven species of malvaceae plants. Volatile organic compounds (VOCs) from the plants were sampled using dynamic headspace adsorption device and then identified and quantified on GC-MS. EAG tests and taxis selection were assayed for the responses of A. lucorum to 11 candidate volatiles from the malvaceae plants. The types and contents of volatiles collected from the seven malvaceae plants were different, and were even different from the four cotton varieties. The total volatile amount was the largest in cotton shiyuan321, with (3E)-4, 8-dimethylnona-1, 3, 7-triene (DMNT) accounting for the most and nonanal and ocimene followed. The types and amount of volatiles in hollyhock Althaea rosea were the least. Eleven candidate volatiles elicited EAG responses in both male and female adult bugs and the responses were stronger in males than in females. The EAG responses were the highest for trans-2-hexenyl butyrate, and strong EAG responses to linalool, leaf acetate, 6-methyl-5-hepten-2-one and DMNT were also observed. In behavior assay, α-pinene, DMNT and limonene attracted male bugs, and γ-terpinene, DMNT, myrcene, humulene, ocimene and leaf acetate attracted female bugs, γ-terpinene and leaf acetate being the best attractant for females. Therefore, male and female bugs showed different EAG and behavior responses to the volatiles from the malvaceae plants. The candidate chemicals were the potential attractants to be developed for control of A. lucorum in field.

Key words: Apolygus lucorum, malvaceae plants, volatiles, EAG response, taxis behavior

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