基因间插入对水稻农艺性状及刺吸式口器害虫取食选择性与种群密度的影响

doi:10.16409/j.cnki.2095-039x.2017.01.003

摘要/Abstract

摘要： 本文评价了T-DNA插入对水稻农艺性状与抗虫性、褐飞虱Nilaparvata lugens Stål取食选择性及3种刺吸式口器害虫种群密度的影响，以分析转基因操作及外源基因插入可能引起的非预期效应。结果表明，供试ZD1640-3、ZD1640-19、ZD1640-23和ZD1640-24共4个突变体株系T-DNA插入位点附近基因的表达均不受影响；除个别株系的株高、每穗粒数或结实率有降低外，主要农艺性状参数与受体亲本无显著差异。除ZD1640-24外，突变体各株系上褐飞虱、白背飞虱Sogatella furcifera（Horváth）和黑尾叶蝉Nephotettix bipunctatus（Fabricius）平均种群密度与受体亲本无显著差异；且4个突变体株系上3种害虫种群密度在大多数情况下与受体亲本也无显著差异。与受体亲本相比，突变体株系ZD1640-24对褐飞虱的抗性及取食选择性无显著影响。综合分析认为，T-DNA基因间插入并没有引起水稻发生显著的非预期变化，个别突变体某些考查指标的变化主要来自于个体差异。

关键词: 水稻, T-DNA插入, 刺吸式口器害虫, 取食选择性, 种群密度, 非预期效应

Abstract: The impacts of T-DNA intergenic insertion on rice agronomic characters, feeding preference and population abundance of sucking insect pests on T-DNA inserted rice mutants were evaluated for unintended effects resulted from transgenic manipulation or gene insertion. The results indicated that the expression of the OPR (12-oxophytodienoate reductase) gene near the insertion site was not affected in all the four mutant lines ZD1640-3, ZD1640-19, ZD1640-23 and ZD1640-24. Major agronomic characters were not significantly different from those of the non-transgenic parent control except for a decrement in plant height, grain number per spike or seed setting rate in several lines. The average population densities of Nilaparvata lugens, Sogatella furcifera and Nephotettix bipunctatus on mutant lines showed no significant difference with those on patent control Zhonghua 11, except that on the mutant line ZD1640-24, average population density of planthoppers was significantly reduced. However, population dynamics of each sucking pests showed no significant difference on mutant lines with those on non-transgenic parent control on most sampling days. In addition, no significant differences in the resistance to N. lugens and feeding preference of N. lugens were found between the mutant line ZD1640-24 and its parent control either. Our results indicate that the variation between mutant lines and non-transgenic parent was more result from the individual variation than from T-DNA insertion.

Key words: rice, T-DNA insertion, sucking-mouthpart insect pests, feeding preference, population density, unintended effects

中图分类号:

S476

汪芳, 党聪, 常学飞, 王龙, 宁铎, 韩乃顺, 叶恭银. 基因间插入对水稻农艺性状及刺吸式口器害虫取食选择性与种群密度的影响[J]. 中国生物防治学报, 2017, 33(1): 18-25.

WANG Fang, DANG Cong, CHANG Xuefei, WANG Long, NING Duo, HAN Naishun, YE Gongyin. Impact of T-DNA Intergenic Insertion on Rice Agronomic Performance,Feeding Preference and Population Abundance of Sucking Insect Pests[J]. journal1, 2017, 33(1): 18-25.

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