生防放线菌ZZ-9颗粒剂的研制及对黄瓜立枯病的防治效果

doi:10.16409/j.cnki.2095-039x.2023.02.044

摘要/Abstract

摘要： 娄彻氏链霉菌Streptomyces rochei ZZ-9是一株有广谱抑菌活性的生防菌株。本研究采用菌丝生长速率法，通过对载体、稳定剂、崩解剂、粘结剂、湿润剂的筛选，确定娄彻氏链霉菌ZZ-9颗粒制剂的最佳配方：菌液与凹凸棒土比例为2:5，碳酸钙1%，氯化镁2%，聚乙烯醇1%，十二烷基苯磺酸钠0.5%。试验结果显示制备的ZZ-9颗粒剂有效活菌数为1.19×10⁸ cfu/g，粒度为93.5%，水分含量2.5%，水中崩解时间96 s，pH值6.35，硬度为10%。盆栽防病试验结果表明，制备的ZZ-9颗粒剂在施用量为3 g/盆时，对黄瓜立枯病的防治果最佳达到76.92%。对黄瓜幼苗生长的促生作用最明显，发芽率、株高、根长和鲜重分别较对照提高了9.34%、29.52%、27.54%、22.96%。

关键词: 娄彻氏链霉菌, 黄瓜, 立枯丝核菌, 颗粒剂, 防治效果

Abstract: Streptomyces rochei ZZ-9 is a biocontrol strain that with broad-spectrum antifungal activity. The mycelium growth rate method was utilized in this work to screen carriers, stabilizers, disintegrants, adhesives, and wetting agents. The optimal formula of Streptomyces rochei ZZ-9 granules were: the ratio of bacterial solution and attapulgite was 2:5, calcium carbonate 1%, magnesium chloride 2%, polyvinyl alcohol 1%, sodium dodecylbenzene sulfonate 0.5%. The effective viable number of ZZ-9, particle size, moisture content, disintegration time in water, pH value and hardness were 1.19×10⁸cfu/g, 93.5%, 2.5%, 96 s, 6.35 and 10%. The results of pot experiment showed that the optimal controlling effect of cucumber Rhizoctonia rot was 76.92% after application of ZZ-9 granule with 3 g/pot. In addition，the Streptomyces rochei ZZ-9 granules presented significant promoting effects in pot experiment. Compared with the control, the germination rate, plant height, root length and fresh weight of cucumber seedlings were increased by 9.34%, 29.52%, 27.54% and 22.96% after application of Streptomyces rochei ZZ-9 granules, respectively.

Key words: Streptomyces rochei, cucumber, Rhizoctonia solani, granule, controlling effect

中图分类号:

S476

张扬, 魏岚菁, 陈军宏, 黑雅娅, 薛应钰. 生防放线菌ZZ-9颗粒剂的研制及对黄瓜立枯病的防治效果[J]. 中国生物防治学报, 2023, 39(5): 1194-1203.

ZHANG Yang, WEI Lanjing, CHEN Junhong, HEI Yaya, XUE Yingyu. Research of Biocontrol Actinomycete ZZ-9 Granules and Its effect on cucumber Rhizoctonia Rot[J]. Chinese Journal of Biological Control, 2023, 39(5): 1194-1203.

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