苯并噻二唑与绿色木霉菌协同施用对三七生长和根腐病的影响

doi:10.16409/j.cnki.2095-039x.2025.02.073

摘要/Abstract

摘要： 本研究通过叶面喷施苯并噻二唑（BTH）与根际接种有益绿色木霉菌Trichoderma viride的协同处理，系统评价了对三七生长和抗病性的提升效果，以期为三七连作障碍的绿色防控提供新思路和新方法。结果显示，绿色木霉菌对三七根腐病主要病原菌黄瓜织球壳菌Plectosphaerella cucumerina和毁灭柱孢菌Cylindrocarpon destructans (RS6)表现出显著的抑制活性，抑制率均在58%以上，而BTH单独处理对根腐病原菌生长无显著影响。盆栽试验表明，绿色木霉菌能在三七根际稳定定殖，当其与BTH联合处理时，可显著降低三七根腐病发病率至37.67%，存苗率提高10.62%，鲜重增加0.34 g，其中0.6 mmol/L BTH为最佳处理浓度，对根腐病的防效为55.29%。此外，0.4 mmol/L BTH与绿色木霉菌联合处理能显著提高三七皂苷含量，包括Rd、R1、Rb₁以及5种皂苷总含量（Re+Rd+R1+Rb₁+Rg1）。综上所述，叶面喷施BTH与根部灌施绿色木霉菌复合处理显著促进三七的生长和皂苷积累，同时提高其抗病性，有效缓解根腐病的发生，为三七连作障碍治理和其他作物病害的生态防控提供了理论依据。

关键词: 三七, 苯并噻二唑, 绿色木霉菌, 根腐病

Abstract: This study systematically evaluated the effects of synergistic treatment with foliar benzothiadiazole (BTH) application and root inoculation of beneficial Trichoderma viride on growth and disease resistance of Panax notoginseng, aiming to provide novel strategies and approaches for green control of replant failure in P. notoginseng cultivation. The results showed that T. viride significantly inhibited the root rot pathogens Plectosphaerella cucumerina (P) and Cylindrocarpon destructans (RS6), with inhibition rates exceeding 58%, while BTH alone had no significant inhibitory effect on the growth of root rot pathogens. Pot experiment demonstrated that T. viride could stably colonize the rhizosphere of P. notoginseng. When co-applied with BTH treatment, this combination significantly reduced the incidence of root rot disease to 37.67%, increased the seedling survival rate by 10.62%, and enhanced the fresh weight by 0.34 g. Notably, 0.6 mmol/L BTH was identified as the optimal concentration, achieving a maximum control efficiency of 55.29% against root rot. Furthermore, combined treatment with 0.4 mmol/L BTH and T. viride significantly increased the content of saponins, including Rd, R1, Rb₁, and the total content of five major saponins (Re+Rd+R1+Rb₁+Rg₁). In conclusion, the synergistic application of foliar BTH spray and root T. viride inoculation significantly promoted the growth and saponin accumulation of P. notoginseng, enhanced its disease resistance, and effectively alleviated root rot occurrence. These findings provided theoretical evidence for the ecological control of continuous cropping obstacles of P. notoginseng and other crop diseases.

Key words: Panax notoginseng, benzothiadiazole, Trichoderma viride, root rot disease

中图分类号:

S476

严秀孛, 李改茴, 罗丽芬, 赵思菡, 金双婷, 尹童, 朱书生, 杨敏. 苯并噻二唑与绿色木霉菌协同施用对三七生长和根腐病的影响[J]. 中国生物防治学报, 2026, 42(1): 44-54.

YAN Xiubei, LI Gaihui, LUO Lifen, ZHAO Sihan, JIN Shuangting, YIN Tong, ZHU Shusheng, YANG Min. Effects of Combined Application of Benzothiadiazole and Trichoderma viride on Growth and Root Rot Occurrence of Panax notoginseng[J]. Chinese Journal of Biological Control, 2026, 42(1): 44-54.

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