谷氏菌素高效生物合成关键基因挖掘及机制研究

doi:10.16409/j.cnki.2095-039x.2026.02.037

摘要/Abstract

摘要： 本研究聚焦于谷氏菌素生物合成基因簇中关键调控靶点的挖掘，通过构建该基因簇中的过表达菌株gouA-gouL、综合表型分析、代谢物检测及蛋白质组学等方法，系统探究了过表达工程菌株的生长、产孢能力、抑菌活性、谷氏菌素产量及其代谢网络的调控效应。结果表明，与野生型相比，过表达乙酰化酶编码基因gouC与过表达甲基转移酶编码基因gouD的菌株可提前产孢，在0～120 h内菌丝生长速率显著提升，对红酵母的抑菌活性分别提高了37.34%和24.33%，谷氏菌素产量提升194.82%和163.28%，分别达2.23和1.99 g/L。蛋白质组学分析表明，基因gouC和gouD过表达能够激活糖代谢和氨基酸代谢途径，并增强UDP-葡糖醛酸前体物质的供应。本研究深化了对谷氏菌素合成调控机制的理解，为构建高产菌株及优化其代谢网络提供了理论依据与科学基础。

关键词: 链霉菌, 谷氏菌素, 蛋白组, 高产改造

Abstract: This study aimed to identify key regulatory elements in the gougerotin biosynthetic gene cluster(BGC) by constructing strains overexpressing the gouA-gouL genes in Streptomyces noourise CK-15. Through comprehensive phenotypic analysis, metabolite detection, and proteomics, the overexpression strains were systematically investigated for their growth, sporulation, antimicrobial activity, gougerotin production, and regulatory effects on the metabolic network. The results demonstrated that strains overexpressing gouC(encoding an acetyltransferase) and gouD(encoding a methyltransferase) exhibited earlier sporulation compared to the wild-type,4 days after cultivation. Mycelial growth rates were significantly higher during the 0–120 h period, and antimicrobial activity against Rhodotorula increased by 37.34% and 24.33%, respectively. Compared to the wild-type, gougerotin production in the gouC and gouD overexpressing strains increased by 194.82% and 163.28%, reaching 2.23 g/L and 1.99 g/L, respectively. Proteomic analysis further revealed that overexpression of these two genes activated carbohydrates and amino acids metabolism pathways and enhanced the supply of UDP-glucuronic acid precursors. This study extends our knowledge of the regulatory mechanisms in gougerotin biosynthesis and provides theoretical and scientific foundations for constructing high-yield strains and optimizing their metabolic networks.

Key words: Streptomyces, gougerotin, proteomics, high-yield modification

中图分类号:

S476

余花, 周倩影, 廖玙彤, 臧政屹, 葛蓓孛. 谷氏菌素高效生物合成关键基因挖掘及机制研究[J]. 中国生物防治学报, 2026, 42(4): 886-897.

YU Hua, ZHOU Qianying, LIAO Yutong, ZANG Zhengyi, GE Beibei. Discovery of Key Genes and Pathway Mechanisms Driving Efficient Gougerotin Biosynthesis[J]. Chinese Journal of Biological Control, 2026, 42(4): 886-897.

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