粉红螺旋聚孢霉NF-06固体发酵条件优化及对南方根结线虫的防治效果

doi:10.16409/j.cnki.2095-039x.2019.06.007

中国生物防治学报 ›› 2020, Vol. 36 ›› Issue (1): 105-112.DOI: 10.16409/j.cnki.2095-039x.2019.06.007

粉红螺旋聚孢霉NF-06固体发酵条件优化及对南方根结线虫的防治效果

张洁¹, 郭雪萍², 夏明聪¹, 孙润红¹, 武超¹, 刘红彦¹, 杨丽荣¹, 张猛²

1. 河南省农业科学院植物保护研究所/河南省生物农药工程研究中心/河南省作物保护国际联合实验室, 郑州 450002;
2. 河南农业大学, 郑州 450002

收稿日期:2019-05-09 出版日期:2020-02-08 发布日期:2020-02-26
通讯作者: 杨丽荣,副研究员,E-mail:luck_ylr@126.com;张猛,教授,E-mail:zm20066@126.com
作者简介:张洁,博士,助理研究员,E-mail:zhangjie656@126.com
基金资助:
河南省科技攻关专项（182102110272）；河南省农业科学院科研发展专项资金（2019CY02）；河南省农业科学院自主创新基金项目（20192C33）

Optimization of Solid State Fermentation Conditions of Clonostachys rosea NF-06 and Its Control Efficiency on Meloidogyne incognita

ZHANG Jie¹, GUO Xueping², XIA Mingcong¹, SUN Runhong¹, WU Chao¹, LIU Hongyan¹, YANG Lirong¹, ZHANG Meng²

1. Henan Biopesticide Engineering Research Center/Henan International Joint Laboratory of Crop Protection/Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China;
2. Henan Agricultural University, Zhengzhou 450002, China

Received:2019-05-09 Online:2020-02-08 Published:2020-02-26

摘要/Abstract

摘要： 南方根结线虫是一种严重危害我国蔬菜安全生产的土传病害，生产上亟需一种有效的防控方法。本研究从根结线虫卵囊中分离到一株生防真菌NF-06，通过形态学和分子生物学方法将其鉴定为粉红螺旋聚孢霉Clonostachys rosea。通过离体活性测定的方法测定NF-06发酵滤液对根结线虫2龄幼虫的致死作用，发现24、48和72 h后2龄幼虫的相对死亡率分别达63.6%，75.5%和87.0%。以产孢量为指标，优化菌株NF-06固体发酵条件，确定最佳基础培养基为麦麸和麦秸秆混合物，其比例为4：1（wt/wt），在基础培养基中添加10%玉米粉、2%蚕豆粉和0.05% KH₂PO₄可获得最大产孢量；最优培养条件为初始pH值7.0，初始含水量40%，接种量5%，产孢量达8.9×10¹⁰ CFU/g。室内盆栽防治效果测定显示，菌株NF-06固体发酵物能够有效降低土壤中的线虫数量和番茄根结指数，其中5 g/株穴施处理的线虫减退率和防治效果分别达67.9%和69.6%。本研究为生物杀线虫剂的开发和利用提供理论依据。

关键词: 根结线虫, 粉红粘帚霉, 生物防治, 发酵条件优化

Abstract: Root-knot nematode Meloidogyne incognita is an important soil-borne disease that threatens the safety of vegetables. Effective control measures are in urgent need. In this study, a biocontrol fungal strain NF-06 was isolated from the egg masses of M. incognita, identified as Clonostachys rosea by morphological and rDNA-ITS sequence analysis. An initial assay of NF-06 culture filtrates on second-stage juveniles was conducted, which showed relative mortalities of 63.6%, 75.5% and 87.0% at 24 h, 48 h and 72 h post treatment, respectively. The solid state fermentation conditions of the strain NF-06 were optimized using the conidium number as an index. The results showed that the optimal basal medium was the mixture of wheat bran and wheat straw, with a mass ratio of 4:1 (wt/wt). When corn flour (10%), broad bean (2%) and KH₂PO₄(0.05%) were supplemented into the basal medium, the maximum conidial production was achieved. The viable conidial number reacheed 8.9×10¹⁰ CFU/g at the optimal culture conditions of initial pH 7.0, initial water content 40%, and inoculum dose 5%. The NF-06 solid culture significantly reduced the population densities of juveniles in soil and root-knot index in tomato in a greenhouse test. When hole applied at 5 g per plant, the NF-06 solid culture afforded reduction rates of juveniles and control efficiency of 67.9% and 69.6%, respectively. This study provides a basis for the research and development of bio-nematicide.

Key words: root-knot nematode, Clonostachys rosea, biological control, fermentation condition optimizition

中图分类号:

S476.1

张洁, 郭雪萍, 夏明聪, 孙润红, 武超, 刘红彦, 杨丽荣, 张猛. 粉红螺旋聚孢霉NF-06固体发酵条件优化及对南方根结线虫的防治效果[J]. 中国生物防治学报, 2020, 36(1): 105-112.

ZHANG Jie, GUO Xueping, XIA Mingcong, SUN Runhong, WU Chao, LIU Hongyan, YANG Lirong, ZHANG Meng. Optimization of Solid State Fermentation Conditions of Clonostachys rosea NF-06 and Its Control Efficiency on Meloidogyne incognita[J]. Chinese Journal Of Biological Control, 2020, 36(1): 105-112.

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粉红螺旋聚孢霉NF-06固体发酵条件优化及对南方根结线虫的防治效果

Optimization of Solid State Fermentation Conditions of Clonostachys rosea NF-06 and Its Control Efficiency on Meloidogyne incognita

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