双纹须歧角螟的求偶和交配与日龄和昼夜节律的关系

doi:10.16409/j.cnki.2095-039x.2025.09.010

摘要/Abstract

摘要： 本研究旨在探究双纹须歧角螟Trichophysetis cretacea Butle雌雄成虫的羽化规律、生理状态、求偶和交配行为节律，以期为性信息素技术的应用提供关键参数。通过在实验室内饲养来自广西横县的幼虫，系统观察其羽化过程、生殖系统发育以及交配行为，并测定性信息素滴度，分析交配行为与日龄和昼夜节律的关系，同时，调查田间性信息素诱捕雄成虫的昼夜活动规律。结果表明：同一批次幼虫中，雌蛾羽化数量显著高于雄蛾，羽化高峰出现在光期的14 h，该时段羽化个体占总数的近50%。雌蛾羽化时卵巢已经发育成熟，雄蛾初羽化时精巢体积最大，随后随着日龄增长逐渐减小，精巢体积（y）与日龄（x）的函数关系为y＝-0.008520x+0.09697。性信息素测定显示：采用浸提法时，性信息素滴度在不同时间点无显著差异；而采用吸附法测定，暗期6 h和8 h为最高，表明雌蛾的求偶和释放性信息素的时间较为集中。交配行为与日龄密切相关，最高交配比例出现在1～3日龄成虫中，占总交配比例的84%。交配行为主要发生在暗期2～6 h。雌雄蛾的交配时间无显著差异，不同日龄个体的交配持续时间也无显著变化。田间性信息素诱捕试验显示，诱捕活动主要集中在暗期第4 h至光期第3 h，其中在暗期第10 h至光期第3 h的诱捕量最高，占总诱捕量的81.5%。因此，在茉莉花生产上采用性信息素监测和防控双纹须歧角螟是一种比较理想的非化学防控措施。当采用主动喷射交配干扰释放器时，喷射时间应该设置为从暗期开始至光期3 h结束。

关键词: 双纹须歧角螟, 求偶, 性信息素滴度, 交配, 昼夜节律, 日龄

Abstract: This study aimed to investigate the emergence patterns, physiological state, calling and mating rhythm of male and female adults of Trichophysetis cretacea Butle, serving to provide key technical parameters for the application of sex pheromone technology. Larvae of T. cretacea were collected in Hengxian County, Guangxi, and their eclosion, development of reproductive system, and mating behavior were systemically observed in the laboratory. Sex pheromone titers were measured and the relationship between mating behavior and age and circadian rhythm were analyzed. Furthermore, the circadian rhythm of male adults trapped by sex pheromone in the field was investigated. From the same colony of T. cretacea larvae, the number of female adults emerged was significantly more than that of male moths. Peak emergence occurred during the 14th hour of the light period, with nearly 50% of emergence. Ovaries matured at the time of emergence, while testis volume was the maximum at initial emergence, decreasing with age. The functional relationship between testis volume (y) and age (x) was y＝-0.008520x+0.09697. Sex pheromone titers measured using the extraction method showed no significant differences between different time points. However, when measured using SPME adsorption method, the highest titers were observed between 6 h and 8 h in the dark period, indicating that the timing for female moths to call and release sex pheromones was more concentrated. Mating behavior is closely related to age, with the highest mating rate occurring in 1- to 3-day-old adults, accounting for 84% of the total mating rate. Mating primarily occurred between 2 h and 6 h in the dark period. There was no significant difference in the mating time between male and female moths, nor was the duration of mating significantly different between age groups. Sex pheromone trap catch was high from 4 h in the dark period to 3 h in the light period, with the peak from 10 h in the dark period to 3 h in the light period, accounting for 81.5% of the total. Therefore, using sex pheromones to monitor and control T. cretacea borers in jasmine crops is an ideal non-chemical control measure. When an active aerosol dispenser for mating disruption is to be used, it is recommended to set the spraying time from the beginning of the dark period to the end of the 3 h light period.

Key words: Trichophysetis cretacea, calling, sex pheromone titer, mating, circadian rhythm, age

中图分类号:

S476

农丽丽, 黄晞, 尹丰平, 檀志全, 黄芳燕, 韦玉全, 杜永均. 双纹须歧角螟的求偶和交配与日龄和昼夜节律的关系[J]. 中国生物防治学报, 2025, 41(6): 1333-1340.

NONG Lili, HUANG Xi, YIN Fengping, TAN Zhiquan, HUANG Fangyan, WEI Yuquan, DU Yongjun. Relationship between Calling and Mating and Age and Circadian Rhythm in Trichophysetis cretacea[J]. Chinese Journal of Biological Control, 2025, 41(6): 1333-1340.

参考文献

[1] 陈殷, 赵勇, 焦世霞, 等. 全国主要茉莉花茶产区概览[J]. 中国茶叶, 2017, 39(6): 37.
[2] 王助引, 周至宏, 韦德卫, 等. 双纹须歧角螟的生物学及防治[J]. 昆虫知识, 2003(4): 340-343.
[3] 王助引, 周至宏, 韦德卫. 茉莉花新害虫——双纹须歧角螟[J]. 广西农业科学, 2001(3): 131-137.
[4] 龚兰芳, 陆星星, 范文红. 茉莉蕾螟的发生规律调查[J]. 植物保护, 2007(5): 127-130.
[5] 李娟, 彭成林, 刘琼英, 等. 不同药剂防治双纹须歧角螟田间药效试验[J]. 中国农业信息, 2015(3): 73.
[6] Peng C L, Gu P, Li J, et al. Identification and field bioassay of the sex pheromone of Trichophysetis cretacea (Lepidoptera: Crambidae)[J]. Journal of Economic Entomology, 2012, 105(5): 1566-1572.
[7] Gu P, Peng C L, Li J, et al. Optimization and field evaluation of the sex pheromone of Trichophysetis cretacea (Lepidoptera, Crambidae) for pest monitoring[J]. Journal of Applied Entomology, 2013, 137(6): 462-468.
[8] He Y Q, Feng B, Guo Q S, et al. Age influences the olfactory profiles of the migratory oriental armyworm Mythimna separate at the molecular level[J]. BMC Genomics, 2017, 18(1): 32.
[9] Gadenne C, Barrozo R B, Anton S. Plasticity in insect olfaction: to smell or not to smell?[J]. Annual Review of Entomology, 2016, 61: 317-333.
[10] Reyes H, Arzuffi R, Robledo N. Effects of male age and mating status on response to the female sex pheromone of Copitarsia decolora (Lepidoptera: Noctuidae)[J]. Florida Entomologist, 2015, 98(1): 47-51.
[11] Lu J F, Yao X M, Shen Y, et al. Physiological status of rice leaf-roller Cnaphalocrocis medinalis (Lepidoptera: Crambidae) adults trapped by sex pheromone and floral odor[J]. Insects, 2024, 15(9): 637.
[12] Brady D, Saviane A, Cappellozza S, et al. The circadian clock in Lepidoptera[J]. Frontiers in Physiology, 2021, 12: 1-18.
[13] Groot A T. Circadian rhythms of sexual activities in moths: a review[J]. Frontiers in Ecology and Evolution, 2014, 2: Article 43.
[14] Aranda-Arguello R, Malo E A, López-Guillen G, et al. Gymnandrosoma aurantianum (Lepidoptera: Tortricidae): Calling rhythm and effect of age on female sex pheromone titer and male antennal responses[J]. Neotropical Entomology, 2024, 53(1): 75-82.
[15] Foster S P, Anderson K G. Some factors influencing calling behavior and mass emission rate of sex pheromone from the gland of the moth Chloridea virescens[J]. Journal of Chemical Ecology, 2022, 48(2): 141-151.
[16] Guo Q S, Yang S J, Zhuo F Y, et al. Effect of age, daily rhythm and mating on pheromone titres and trapping of Glyphodes pyloalis (Lepidoptera,Crambidae)[J]. Journal of Applied Entomology, 2022, 146(7): 875-884.
[17] Pham H T, McNamara K B, Elgar M A. Age-dependent chemical signalling and its consequences for mate attraction in the gumleaf skeletonizer moth[J]. Animal Behaviour, 2021, 173: 207-213.
[18] Jiménez-Pérez A, Wang Q, Arzuffi R. Mating Behavior of Cnephasia jactatana (Lepidoptera: Tortricidae), an important pest of Kiwifruit[J]. Journal of Economic Entomology, 2013, 106(3): 1373-1378.
[19] Kong W N, Wang Y, Li N, et al. Variations in mating and reproduction in oriental fruit moth caused by adult physiological state in laboratory tests[J]. Insects, 2024, 15(6): 457.
[20] Levi-Zada A, Byers J A. Circadian rhythms of insect pheromone titer, calling, emission, and response: a review[J]. Science of Nature, 2021, 108(5): 35.
[21] 施乾芮, 董义霞, 聂珍, 等. 番茄潜叶蛾成虫运动、求偶和交配行为的昼夜节律[J]. 昆虫学报, 2024, 67(3): 393-403.
[22] 冯波, 郭前爽, 姚晓明, 等. 二化螟雄蛾交配行为与精巢大小的关系[J]. 昆虫学报, 2019, 62(7): 849-856.
[23] 冯波, 刘天伟, 陆明红, 等. 二化螟的多次交配及其对雌蛾产卵量的影响[J]. 昆虫学报, 2020, 63(6): 759-768.
[24] Kanno H, Sato A. Mating-behavior of rice stem borer moth, Chilo suppressalis Walker (Lepidoptera-Pyralidae).1. Effects of moth age on mating activity[J]. Applied Entomology and Zoology, 1978, 13: 215-221.
[25] Hou M L, Sheng C F. Fecundity and longevity of Helicoverpa armigera (Lepidoptera: Noctuidae): Effects of multiple mating[J]. Journal of Economic Entomology, 1999, 92(3): 569-573.
[26] Hou M L, Sheng C F. Calling behaviour of adult female Helicoverpa armigera (Hubner) (Lep., Noctuidae) of overwintering generation and effects of mating[J]. Journal of Applied Entomology-Zeitschrift Fur Angewandte Entomologie, 2000, 124(2): 71-75.
[27] Greenway E V, Cirino L A, Wilner D, et al. Extreme variation in testes size in an insect is linked to recent mating activity[J]. Journal of Evolutionary Biology, 2020, 33, 142-150.
[28] Sasson D A, Munoz P R, Gezan S A, et al. Resource quality affects weapon and testis size and the ability of these traits to respond to selection in the leaf-footed cactus bug, Narnia femorata[J]. Ecology and Evolution, 2016, 6: 2098-2108.
[29] Hiroyoshi S, Mitsunaga T, Reddy G P. Effects of temperature, age and stage on testis development in diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae)[J]. Physiological Entomology, 2021, 46: 200-209.
[30] Huang J, Wu E, Yang C, et al. Mating disruption of the tomato leafminer Tuta absoluta (Lepidoptera: Gelechiidae) on greenhouse tomatoes[J]. Crop Health, 2024, 2(1): 15.
[31] Vacas S, Navarro I, Primo J, et al. Mating disruption to control the striped rice stem borer: Pheromone blend, dispensing technology and number of releasing points[J]. Journal of Asia-Pacific Entomology, 2016, 19(2): 253-259.