长枝木霉菌与贝莱斯芽孢杆菌协同防治甘蔗赤腐病

doi:10.16409/j.cnki.2095-039x.2026.02.001

摘要/Abstract

摘要： 为明确长枝木霉菌SOR-1与贝莱斯芽孢杆菌YC89对甘蔗赤腐病的协同防治效果。本研究采用菌丝生长速率法对共培养体系的菌株添加顺序和发酵时长进行探究，并通过盆栽试验测定施用共培养发酵液（COYS）对赤腐病的防治效果以及对甘蔗叶片相关防御酶活性的影响。结果表明，菌株YC89与SOR-1联合发酵5 d的共培养模式对镰孢炭疽菌具有显著的抑菌效果； COYS对甘蔗赤腐病的防治效果最好，为62.25%，较SOR-1和YC89单培养发酵液及复配发酵液YC89+SOR-1的防效分别提高了20.41%、10.21%和2.05%。此外，COYS处理的甘蔗叶片POD、CAT、PAL、SOD活性明显高于CK，峰值分别是CK的3.02、5.45、3.63和2.60倍。综上，SOR-1和YC89共培养发酵液对甘蔗赤腐病的防治效果优于单培养，为生防菌联合防治甘蔗赤腐病提供了理论基础。

关键词: 甘蔗赤腐病, 长枝木霉菌, 贝莱斯芽孢杆菌, 共培养, 协同防治

Abstract: In order to clarify the synergistic control effect of Trichoderma longibrachiatum SOR-1 and Bacillus velezensis YC89 against sugarcane red rot, explored the sequence of strain addition and fermentation time in the co-culture system by.using mycelial growth rate method. Then, pot experiments were conducted to evaluate the control efficacy of the co-culture fermentation broth (COYS) against sugarcane red rot and its impact on the activity of defense-related enzymes in leaves. The results showed that the treatment sequence of first inoculating YC89 followed by SOR-1 and co-culturing for 5 days exhibited the strongest inhibitory effect against Fusarium oxysporum. The control effect of COYS on sugarcane red rot is the best, at 62.25%, which is 20.41%, 10.21%, and 2.05% higher than the control effects of SOR-1 and YC89 single culture fermentation broth and compound fermentation broth YC89+SOR-1, respectively. Additionally, the activities of POD, CAT, PAL, and SOD in sugarcane leaves treated with COYS were significantly higher than those in the control group (CK), with peak values being 3.02, 5.45, 3.63 and 2.60 times that of CK, respectively. In conclusion, the co-cultured fermentation both of SOR-1 and YC89 demonstrated superior disease control efficacy compared to single-cultured broths, which provides a theoretical basis for the synergistic biological control of sugarcane red rot disease.

Key words: sugarcane red rot disease, Trichoderma longibrachiatumlong, Bacillus velezensis, co-culture, Collaborative prevention and control

中图分类号:

S476

董玲玲, 张建萍, 何狄霏, 李先丽, 曹红钰, 李亚铭, 何丽莲, 李富生. 长枝木霉菌与贝莱斯芽孢杆菌协同防治甘蔗赤腐病[J]. 中国生物防治学报, 2026, 42(1): 55-63.

DONG Lingling, ZHANG Jianping, HE Difei, LI Xianli, CAO Hongyu, LI Yaming, HE Lilian, LI Fusheng. Synergistic Control of Trichoderma longibrachiatum and Bacillus velezensis against Sugarcane Red Rot[J]. Chinese Journal of Biological Control, 2026, 42(1): 55-63.

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