生防黑附球菌En13的抗真菌活性分析

doi:10.16409/j.cnki.2095-039x.2025.07.009

摘要/Abstract

摘要： 黑附球菌Epicoccum nigrum是一类研究较少的生防真菌，其可以分泌多种抗真菌代谢产物，用于植物病害的生物防治。本研究从猕猴桃叶片中分离了一系列内生真菌，通过平板拮抗和猕猴桃接种，筛选到了一株可以抑制猕猴桃真菌性软腐病发生的生防真菌。通过ITS系统发育树分析将其鉴定为黑附球菌，命名为En13。在此基础上，本研究对En13的生防活性进行进一步评估。通过平板对峙培养发现En13对链格孢菌、尖孢镰刀菌、稻瘟病菌均具有拮抗效果，说明En13具有广谱抗真菌活性。对En13的防病效果进行评估，发现其可以高效抑制链格孢菌对烟草以及稻瘟病菌对水稻的侵染，具有良好的生防潜力。对En13进行基因组测序分析，发现其基因组中编码大量次生代谢产物合成基因簇，包括嗜铁素（tolypyridone C）、aspirochlorine等抗菌代谢产物，说明其可能是通过分泌抗菌代谢产物发挥生防功能。通过分析En13在不同培养基上的生长速度和产孢量，发现黄豆浆培养基是En13的最佳生长与产孢培养基。

关键词: 黑附球菌, 平板拮抗, 生物防治

Abstract: Epicoccum nigrum can secrete antifungal metabolites and control plant fungal disease, yet it is poorly studied. Through paired culture and inoculation of endophytic fungi isolated from kiwifruit leaves on kiwifruit, we found a fungus strain No.13 that could protect kiwifruit from fruit rot. By phylogenetic tree analysis based on ITS sequence, the No.13 strain was identified as Epicoccum nigrum and named as En13. To investigate the antifungal spectrum of En13, we analyzed its biocontrol activity to other pathogenic fungi. En13 showed antagonistic activity to Alternaria alternata, Fusarium oxysporum and Magnaporthe oryzae during paired culture, indicating that the antifungal activity of En13 was broad-spectrum. By co-inoculating on plants, we found En13 could inhibit A. alternata infection of tobacco and M. oryzae infection of rice. By genome sequencing, we found the genome of En13 encoded many secondary metabolism gene clusters, including antifungal metabolites metachelin C and aspirochlorine, indicating that En13 inhibits fungi by secreting antifungal metabolites. At last, we screened different media and found soybean medium is the best medium for mycelial growth and conidia production.

Key words: Epicoccum nigrum, antagonistic activity, biocontrol

中图分类号:

S476.2

林龙, 管芸, 薛畅达, 高鹏龙, 王平平, 崔传斌, 钱国良. 生防黑附球菌En13的抗真菌活性分析[J]. 中国生物防治学报, 2026, 42(2): 503-511.

LIN Long, GUAN Yun, XUE Changda, GAO Penglong, WANG Pingping, CUI Chuanbin, QIAN Guoliang. Antifungal Activity Characterization of Biocontrol Fungus Epicoccum nigrum En13[J]. Chinese Journal of Biological Control, 2026, 42(2): 503-511.

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