解淀粉芽孢杆菌BA-12可湿性粉剂研制及对核桃根腐病的防治效果

doi:10.16409/j.cnki.2095-039x.2016.05.011

摘要/Abstract

摘要： 为获得对核桃根腐病具有优良防治效果的解淀粉芽孢杆菌BA-12可湿性粉剂，采用单因素法和正交试验法筛选出对菌株BA-12活性影响最小和悬浮率最佳的载体和助剂及其配比。可湿性粉剂的配方为解淀粉芽孢杆菌10%，载体高岭土30%，湿润剂十二烷基苯磺酸钠10%、分散剂三聚磷酸钠5%、保护剂腐殖酸2.5%。主要性能检测结果表明可湿性粉剂活菌含量为1×10⁸ cfu/g，湿润时间为40 s，悬浮率为79%，pH 6.72，含水率2.61%。稳定性测定中，菌剂对温度、酸碱、紫外光耐受性强，30～45℃、pH 6～8、紫外照射20～30 min均对菌剂影响小。盆栽试验得到500～1000倍稀释的菌剂为防治核桃根腐病推荐使用浓度。

关键词: 解淀粉芽孢杆菌, 可湿性粉剂, 核桃根腐病, 防治效果

Abstract: The single factor and orthogonal test methods were used to screen the carriers, additives and their proportioning to obtain Bacillus amyloliquefaciens BA-12 wettable powder with excellent control effect on walnut root rot. The B. amyloliquefaciens BA-12 wettable powder prescription were comprised 10% B. amyloliquefaciens, 30% the carrier kaolin, 10% sodium dodecyl benzene sulfonate, 5% dispersing agent sodium tripolyphosphate, 2.5% protecting agent humic acid, with the content of living bacterium was 1×10⁸ cfu/g. Its wetting time was 40 s, suspension rate was 79%, pH 6.72, rate of water content was 2.61%. The B. amyloliquefaciens BA-12 wettable powder were stable under the conditions of 30-45℃, pH 6-8 and ultraviolet irradiation 20-30 min. The 500-1000 times diluents on walnut root rot was the recommended concentration in potted tests.

Key words: Bacillus amyloliquefaciens, wettable powder, walnut root rot, control effect

中图分类号:

S476

李姝江, 方馨玫, 曾艳玲, 樊苏恒, 朱天辉. 解淀粉芽孢杆菌BA-12可湿性粉剂研制及对核桃根腐病的防治效果[J]. 中国生物防治学报, 2016, 32(5): 619-626.

LI Shujiang, FANG Xinmei, ZENG Yanling, FAN Suheng, ZHU Tianhui. Preparation of Wettable Powder of Bacillus amyloliquefaciens BA-12 and Its Control Effect on Walnut Root Rot[J]. journal1, 2016, 32(5): 619-626.

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