油茶炭疽菌侵染对油茶叶片内生细菌群落结构的影响

doi:10.16409/j.cnki.2095-039x.2022.04.015

摘要/Abstract

摘要： 植物内生菌与植物长期共存,二者协同进化,互惠互利。在胁迫因素的影响下,内生菌群落组成会发生相应的变化并对植物起到重要的保护作用。然而,关于病原菌胁迫对油茶树叶片内生细菌群落结构的影响尚缺乏系统的研究。为了解云南油茶受炭疽病菌侵染后,叶片内生微生物群落如何参与植物防御,本文基于高通量测序技术,对炭疽菌生物胁迫下云南油茶病、健叶片内生菌群的群落组成和多样性进行分析;基于Bugbase软件对菌群的表型进行预测。结果表明,炭疽菌侵染后显著提高了Chao1、ACE丰富度指数,而多样性指数Simpson、Shannon则无显著变化。门水平上,病、健叶片上相对丰度大于1%的种类相同,但所有门的丰度都表现为病叶高于健叶。属水平上,病叶片中Methylobacterium(1.91%)属相对丰度最高,健康叶片中Pontibacter(1.41%)相对丰度最高。感病叶片中有5个特有属,健康叶片中有3个特有属。Bugbase菌群功能预测表明,云南油茶感病叶片内生菌菌群生物膜形成能力和氧化胁迫耐受增强;革兰氏阳性菌的相对丰度增加,革兰氏阴性菌相对丰度降低。可见,油茶炭疽病的发生改变了油茶树叶片内生细菌的群落结构,使少数抗病相关物种优势生长。研究结果为油茶炭疽病的生物防治提供了信息。

关键词: 炭疽病菌, 高通量测序, 内生细菌, 群落结构, 云南油茶叶片

Abstract: Endophytes and plants coexist for a long time,and they co-evolve for mutual benefits.Under the influence of stress factors,the community composition of endophytic bacteria will change accordingly and play an important role in plant protection.However,the effects of pathogen stress on the community structure of endophytic bacterial in Camellia oleifera leaves has not been systematically investigated.In order to understand how the endophytic microbial community in leaves of Camellia anthracnose participates in plant defense after being infected by anthrax,the community composition and diversity of the endophytic bacteria in the diseased and healthy leaves of C.oleifera in Yunnan were analyzed via high-throughput sequencing technology.Alpha diversity analysis showed that the richness index of Chao1 and ACE were significantly increased after infection by anthracnose,while the diversity index of Simpson and Shannon were not significantly changed.At the phylum level,the phyla with a relative abundance more than 1% in diseased and healthy leaves were same,but the abundance in diseased leaves was higher than that in healthy leaves.At the genus level,Methylobacterium(1.91%) was dominant in diseased leaves,and Pontibacter(1.41%) was dominant in healthy leaves.There were 5 special genera in diseased leaves,and 3 special genera in healthy leaves.Bugbase functional prediction showed that the biofilm-producing ability and oxidative stress tolerance of the endophytic bacteria in the diseased leaves were enhanced,the relative abundance of gram-positive bacteria was increased and that of gram-negative bacteria were decreased.This paper suggests that the occurrence of C.oleifera anthracnose changed the community structure of endophytic bacteria in C.oleifera leaves,allowing a few disease-resistant related species to grow dominantly.The research is of significance in biological control of C.oleifera anthracnose.

Key words: anthracnose, high-throughput sequencing, endophytic bacteria, community structure, Yunnan Camellia oleifera leaf

中图分类号:

S476

崔明秦, 张东华, 闫晓慧, 洪英娣, 马焕成, 伍建榕, 刘丽. 油茶炭疽菌侵染对油茶叶片内生细菌群落结构的影响[J]. 中国生物防治学报, 2022, 38(4): 911-919.

CUI Mingqin, ZHANG Donghua, YAN Xiaohui, HONG Yingdi, MA Huancheng, WU Jianrong, LIU Li. Effects of Colletotrichum sp.Infection on Endophytic Bacterial Community in Leaves of Camellia oleifera[J]. Chinese Journal of Biological Control, 2022, 38(4): 911-919.

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