草地贪夜蛾肠道普罗威登斯菌对氯虫苯甲酰胺的解毒作用

doi:10.16409/j.cnki.2095-039x.2025.09.022

摘要/Abstract

摘要： 氯虫苯甲酰胺是防治草地贪夜蛾害虫常用的化学药剂之一。长期使用会使草地贪夜蛾对氯虫苯甲酰胺产生抗性，降低用药效果。为进一步探究肠道微生物在抗性产生过程中的作用，本研究通过比较取食氯虫苯甲酰胺前后肠道微生物差异，筛选分离差异肠道微生物，并进行生测试验。结果表明：从草地贪夜蛾肠道中筛选得到3种细菌，并通过体外培养法在LB上分离培养，生测发现一菌株能够降低氯虫苯甲酰胺毒力，显著减少草地贪夜蛾的死亡率。通过LC-MS分析氯虫苯甲酰胺和该菌株混合前后量的变化，发现该菌株能够降解氯虫苯甲酰胺，其降解率可达42%。进一步对该菌株进行基因组测序，并构建进化树，鉴定该菌为普罗威登斯菌Providencia rettgeri，基因组大小为4391370 bp，编码3985个基因。综上所述，普罗威登斯菌可以通过直接降解氯虫苯甲酰胺，从而增加草地贪夜蛾对氯虫苯甲酰胺的抗性，为进一步解析草地贪夜蛾对氯虫苯甲酰胺产生抗性的机理提供依据。

关键词: 解毒, 普罗威登斯菌, 氯虫苯甲酰胺, 草地贪夜蛾

Abstract: Chlorantraniliprole(CAP) is one of the commonly employed chemical agents for controlling fall armyworm(FAW). Long-term use will cause the fall armyworm to develop resistance to chlorantranamide, reducing the efficacy of the insecticide. To explore the role of gut microbiota in the process of resistance development, this study compared the differences in intestinal microbiota before and after the intake of chlorantraniliprole, screened and isolated the differential gut microbiota, and conducted bioassay tests. Three types of bacteria were screened and isolated from fall armyworm midgut and cultured on LB by in vitro culture method. Biotests revealed that the strains could reduce the virulence of chlorantraniliprole and significantly reduce the mortality rate of the fall armyworm. The amounts of chlorantraniliprole before and after mixing with the strains were analyzed by LC-MS. It was found that the strains could degrade chlorantraniliprole, with a rate reaching 42%. Further genomic sequencing and phylogenetic analysis identified the strain as Providencia rettgeri, with a genome size of 4391370 bp encoding 3985 genes. In conclusion, P. rettgeri can increase the resistance of fall armyworm to chlorantraniliprole by directly degrading it, providing a basis for further understanding the mechanism on developing resistance to chlorantraniliprole.

Key words: detoxification, Providencia rettgeri, chlorantraniliprole, Spodoptera frugiperda

中图分类号:

S435.6

陈凤, 张峻滔, 王秀平, 张永军, 张天涛. 草地贪夜蛾肠道普罗威登斯菌对氯虫苯甲酰胺的解毒作用[J]. 中国生物防治学报, 2026, 42(4): 809-817.

CHEN Feng, ZHANG Juntao, WANG Xiuping, ZHANG Yongjun, ZHANG Tiantao. Detoxification Effect of Providencia rettgeri in the Gut of Spodoptera frugiperda on Chlorantraniliprole[J]. Chinese Journal of Biological Control, 2026, 42(4): 809-817.

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