MbMNPV对不同群体草地贪夜蛾的毒力差异分析

doi:10.16409/j.cnki.2095-039x.2022.11.028

摘要/Abstract

摘要： 甘蓝夜蛾核型多角体病毒Mamestra brassicae multiple nucleopolyhedrovirus（MbMNPV）是一种广谱昆虫病毒，近年来已被应用于草地贪夜蛾Spodoptera frugiperda J. E. Smith的防治上，而不同地区的草地贪夜蛾因环境因子的差异导致其对MbMNPV等生物农药的敏感性不同。本研究首先基于COI和Tpi基因对湖南长沙、河南济源、四川成都、广西南宁和辽宁丹东等5个地区的草地贪夜蛾群体进行了生物型鉴定，同时通过饲毒法测定了不同群体草地贪夜蛾幼虫对MbMNPV的敏感性。结果表明，基于COI基因的鉴定结果显示湖南长沙、河南济源和辽宁丹东的草地贪夜蛾均为水稻型，四川成都和广西南宁群体的草地贪夜蛾为水稻型和玉米型的混合群体，而基于Tpi基因的鉴定结果显示5个群体的草地贪夜蛾均为玉米型。5个群体的草地贪夜蛾3龄幼虫在浓度为1×10⁸PIB/mL和1×10⁷ PIB/mL的MbMNPV处理下7 d校正死亡率均在90%以上，而浓度为1×10⁶ PIB/mL时，湖南长沙、广西南宁和四川成都群体的校正死亡率显著高于辽宁丹东群体。进一步的毒力测定发现，辽宁丹东群体的LC₅₀是湖南长沙群体的4.9倍，相同浓度下的LT₅₀也均高于湖南长沙群体，同时基于Log-rank检验结果也表明在1×10⁵ PIB/mL～1×10⁸PIB/mL处理下辽宁丹东群体草地贪夜蛾幼虫的平均存活时间显著高于湖南长沙群体。本研究表明，不同地区的草地贪夜蛾群体对MbMNPV敏感性存在差异，辽宁丹东的草地贪夜蛾群体较其他群体对MbMNPV敏感性更低。

关键词: 草地贪夜蛾, 甘蓝夜蛾核型多角体病毒, 生物型, 敏感性

Abstract: Mamestra brassicae multiple nucleopolyhedrovirus (MbMNPV) is a broad-spectrum insect virus, which has been used to control Spodoptera frugiperda J. E. Smith in recent years. However, S. frugiperda populations from different regions have shown varying sensitivity to biological pesticides such as MbMNPV due to environmental factors. In this study, with identification of biotypes of the S. frugiperda populations from Changsha, Hunan (HNCS), Jiyuan, Henan (HNJY), Chengdu, Sichuan (SCCD), Nanning, Guangxi (GXNN), and Dandong, Liaoning (LNDD) by COI and Tpi genes, we determined the sensitivity of S. frugiperda larval populations to MbMNPV by diet method. The identification based on the COI gene showed that the populations of HNCS, LNDD and HNJY were all rice strains and the populations of SCCD and GXNN were mixed corn and rice strain, while the identification based on the Tpi gene showed that all the five populations were corn strains. The mortalities of the five S. frugiperda larval populations were all over 90% with 1×10⁸ PIB/mLand 1×10⁷ PIB/mL of MbMNPV, but with 1×10⁶ PIB/mL of MbMNPV, the mortalities in HNCS, GXNN and SCCD were significantly higher than that in LNDD. The LC₅₀ of LNDD was 4.9 times higher than that of HNCS, and the LT₅₀and mean survival time of LNDD were higher than those of HNCS at the same concentration of MbMNPV. This study shows that there are differences in sensitivity to MbMNPV among populations of S. frugiperda from different regions, S. frugiperda population in LNDD is less sensitive to MbMNPV than the other populations.

Key words: Spodoptera frugiperda, MbMNPV, biotype, sensitivity

中图分类号:

S476.1

史子涵, 王玉生, 李靖, 李有志, 谭琳. MbMNPV对不同群体草地贪夜蛾的毒力差异分析[J]. 中国生物防治学报, 2023, 39(6): 1309-1317.

SHI Zihan, WANG Yusheng, LI Jing, LI Youzhi, TAN Lin. Virulence of MbMNPV to Populations of Spodoptera frugiperda[J]. Chinese Journal of Biological Control, 2023, 39(6): 1309-1317.

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