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Cultural Characteristics and Virulence of Metarhizium spp. to the 2nd Instar Nymphs of Bemisia tabaci

LI Maoye1, CHEN Dexin1,2, LI Shiguang1, LIU Su1, LI Yi1, QIAN Xiaoxiao1, LIN Huafeng1   

  1. 1. School of Plant Protection, Anhui Agricultural University, Hefei 230036, China;2. Qingzhou Tobacco Research Institute, China National Tobacco Corporation, Qingdao 266001, China
  • Received:2014-05-06 Revised:1900-01-01 Online:2015-02-08 Published:2015-02-08

Abstract: Bemisia tabaci (Gennadius) is an important insect pest in China and has developed high resistance to many kinds of insecticides. In this study, 8 strains of Metarhizium spp., isolated from different hosts obtained from various geographical locations were evaluated for their virulence against the 2nd instar nymphs of the “Q-biotype” B. tabaci. The results show that the M. flavoviride strain Mf96 had the highest virulence to the B. tabaci nymphs, with a corrected mortality of 89.5% at 7 d postr treatment and a LT50 value of 3.65 d at the concentration of 1.0×108 conidia/mL. With sprays at 4 concentrations (1.0×108, 1.0×107, 1.0×106 and 1.0×105 conidia/mL) of Mf96 conidial suspension, mortalities of the B. tabaci nymphs increased with treatment concentration and post-treatment time, and the LC50 value was 1.20×107 conidia/mL. Analysis of extracellular protease activities of the 8 strains reveals that enzyme activity (3.64 U/mL) of Mf96 was the highest among all the tested fungal strains. On SDAY medium, Mf96 showed such advantages as fast growth, quick germination and high sporulation, compared to other tested fungal strains. Therefore, Mf96 has a great potential in the biological control of the B. tabaci.

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