Optimizing the Culture Conditions and Research on Stabilities of Antagonistic Substance Produced by Antagonistic Bacteria B-401 aganist Phoma foveata

doi:10.16409/j.cnki.2095-039x.2016.03.012

Abstract

Abstract: UsingPhoma foveata as target pathogen, we studied the optimum culture conditions and stabilities of inhibitive substances secreted by antagonistic endophytic bacteria B-401 from alpine meadow pasture. The results showed that the optimal medium of antagonistic substances produced by B-401 was D broth (beef extract 8 g, yeast extract 5.0 g, glucose 10 g, water 1000 mL), the optimum culture conditions were temperature 23.4 ℃, pH 7, loading volume 35.2 mL/150 mL flask, the way of cultivation was shaking culture under 24 h illumination, the optimum fermentation time was 96 h. The antifungal substances secreted by B-401 were stable at 25―90 ℃, and the antifungal activities were all surpassed over 75%, and it was reduced after treatment at 100 ℃ or more. Also the relative activities were resistant to the metal ions Na⁺, Mg²⁺ and Mn²⁺, and become less stable to Ag⁺, Cu²⁺, Zn²⁺, Fe³⁺.

Key words: Phoma foveata, endophytes of grasses, inhibitive substance, culture conditions, stability

CLC Number:

S476

CHANG Tao, FENG Zhonghong, YANG Chengde, XUE Li. Optimizing the Culture Conditions and Research on Stabilities of Antagonistic Substance Produced by Antagonistic Bacteria B-401 aganist Phoma foveata[J]. journal1, 2016, 32(3): 365-371.

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