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journal1 ›› 2016, Vol. 32 ›› Issue (3): 365-371.DOI: 10.16409/j.cnki.2095-039x.2016.03.012

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Optimizing the Culture Conditions and Research on Stabilities of Antagonistic Substance Produced by Antagonistic Bacteria B-401 aganist Phoma foveata

CHANG Tao, FENG Zhonghong, YANG Chengde, XUE Li   

  1. College of Pratacultural Science, Gansu Agricultural University/Key Laboratory of Grassland Ecosystem Gansu Agricultural University, Ministry of Education/Pratacultural Engineering Laboratory of Gansu Province/Sino-U.S. Center for Grazingland Ecosystem Sustainability, Lanzhou 730070, China
  • Received:2015-10-26 Online:2016-06-08 Published:2016-06-07

Abstract: UsingPhoma foveata as target pathogen, we studied the optimum culture conditions and stabilities of inhibitive substances secreted by antagonistic endophytic bacteria B-401 from alpine meadow pasture. The results showed that the optimal medium of antagonistic substances produced by B-401 was D broth (beef extract 8 g, yeast extract 5.0 g, glucose 10 g, water 1000 mL), the optimum culture conditions were temperature 23.4 ℃, pH 7, loading volume 35.2 mL/150 mL flask, the way of cultivation was shaking culture under 24 h illumination, the optimum fermentation time was 96 h. The antifungal substances secreted by B-401 were stable at 25―90 ℃, and the antifungal activities were all surpassed over 75%, and it was reduced after treatment at 100 ℃ or more. Also the relative activities were resistant to the metal ions Na+, Mg2+ and Mn2+, and become less stable to Ag+, Cu2+, Zn2+, Fe3+.

Key words: Phoma foveata, endophytes of grasses, inhibitive substance, culture conditions, stability

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