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journal1 ›› 2017, Vol. 33 ›› Issue (4): 561-570.DOI: 10.16409/j.cnki.2095-039x.2017.04.018

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Optimizing Fermentation Condition for Bacillus amyloliquefaciens JDF-6 and Analysis of Antifungal Proteins

HU Fei, HU Benjin, ZHOU Ziyan, XU Lina, SU Weihua, SU Xianyan   

  1. Institute of Plant Protection and Agro-products Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, China
  • Received:2016-12-09 Online:2017-08-08 Published:2017-08-11

Abstract: To investigate the best condition for liquid fermentation of Bacillus amyloliquefaciens JDF-6 and the characteristics of antifungal proteins, the liquid fermentation medium and fermentation conditions were optimized by the single factor experiments and orthogonal experiments; crude proteins were extracted by using ammonium sulphate precipitation, and the protease stability, light stability and thermal stability were determined. Results indicated that the optimum medium for Bacillus amyloliquefaciens JDF-6 was 3.0% sucrose, 1% yeast extract powder, 2.0% oil-tea-cake, 0.3% MnSO4. The optimal fermentation conditions were fermentation time for120 h, temperature at 30℃, pH at 6.5-7.0, 5% inoculation quantity, with 10 mL/250 mL medium capacity. Inhibitory activity of crude proteins from strain JDF-6 increased 51.6%, under the optimum fermentation medium and culture conditions. Crude proteins which extracted from strain JDF-6 by using 50% ammonium sulphate for precipitation were sensitive to proteinase, high temperature (>100℃) and UV light (>8 h), with inhibitory activity decreased.

Key words: Bacillus amyloliquefaciens, crude proteins, fermentation, optimize, stability

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