Colonization of Antagonistic Endophytic Bacteria Bacillus mojavensis ZA1 in the Root of Potato and Rhizosphere Soil and Its Effect on Soil Microorganism

doi:10.16409/j.cnki.2095-039x.2016.03.013

Abstract

Abstract: GFP labelling technique and plate culture method were used to study the colonization of endophytic bacterium Bacillus mojavensis ZA1 in the root of potato and rhizosphere soil and its effect on soil microorganism. The results showed that ZA1-gfp colonized well in soil with a density of 3.7×10⁴ to 7.3×10⁴ cfu/g and colonized in the roots of potato and rhizosphere with densities of 49 to 630 cfu/g and 3.0×10³ to 1.5×10⁵ cfu/g, respectively. ZA1-gfp could be detected in inner roots and on the root hairs and root epidermis of potato under a fluorescence microscope. The number of soil bacteria and actinomycetes were increased significantly (P<0.05) after ZA1-gfp inoculation using root-drenching method, but fungi was decreased in 25 days later after ZA1-gfp inoculation; the number of soil bacteria, fungi and actinomycetes was kept well (There was no obvious difference). The results showed that ZA1-gfp could steadily colonize the roots of potato and rhizosphere and the community stability of soil microorganisms was kept well.

Key words: antagonistic endophytic bacteria, GFP labeled, potato, colonization dynamics, soil microorganism

CLC Number:

S476

Wang Ying, Yang Chengde, Xue Li, Cui Yuezhen, Yang Xiaoli. Colonization of Antagonistic Endophytic Bacteria Bacillus mojavensis ZA1 in the Root of Potato and Rhizosphere Soil and Its Effect on Soil Microorganism[J]. journal1, 2016, 32(3): 372-378.

References

[1] 何玲敏, 叶建仁. 植物内生细菌及其生防作用研究进展[J]. 南京林业大学学报(自然科学版), 2014, 38(6): 153-158.
[2] Manoj K S, Chanda K, Ramesh K S. Studies on endophytic colonization ability of two upland rice endophytes, Rhizobium sp. and Burkholderia sp., using green fluorescent protein reporter[J]. Current Microbiology, 2009, 59(3): 240-243.
[3] Thoma P, Sekhar A C. Live cell imaging reveals extensive intracellular cytoplasmic conlonization of banana by normally ono-cultivable endophytic bacteria[J]. AoB Plants, 2014, 6: plu002, doi:10.1093/aobpla/plu002.
[4] Liu B, Qiao H, Huang L, et al. Biological control of take-all in wheat by endophytic Bacillus subtilis E1R-j and potential mode of action[J]. Biological Control, 2009, 49(3): 277-285.
[5] Gao X, Han Q, Chen Y, et al. Biological control of oil seed rape Sclerotinia stem rot by Bacillus subtilis strain Em7[J]. Biocontrol Science and Technology, 2014, 24(1): 39-52.
[6] 畅涛, 王涵琦, 杨成德, 等. 高寒草地禾草内生细菌B-401的鉴定及生物防治潜力评价[J]. 草业学报, 2014, 23(3): 282-289.
[7] Barraquio W L, Revilla L, Ladha J. Isolation of endophytic diazotrophic bacteria from wetland rice[J]. Plant and Soil, 1997, 194(1): 15-24.
[8] Liu X, Jia J, Atkinson S, et al. Biocontrol potential of an endophytic Serratia sp. G3 and its mode of action[J]. World Journal of Microbiology Biotechnology, 2010, 26(8): 1465-1471.
[9] Zhang S M, Sha C Q, Wang Y X, et al. Isolation and characterization of antifungal endophytic bacteria from soybean[J]. Microbiology, 2008, 35(10): 1593-1599.
[10] 王颖, 王玉琴, 杨成德, 等. 马铃薯贮藏期主要病害拮抗内生细菌的筛选、鉴定及功能评价[J]. 草业学报, 2014, 23 (3): 269-275.
[11] 蒋晓玲, 何鹏飞, 王娅玲, 等. 玉米内生细菌Y19的荧光标记及其在玉米体内的定殖应用效果[J]. 玉米科学, 2015, 23(3): 50-56.
[12] 秦楠, 郝林, 李鑫. 解淀粉芽胞杆菌HRH317抗菌蛋白的分离纯化及其抗菌作用[J]. 植物保护学报, 2015, 42(5): 813-819.
[13] Swarnalatha Y, Saha B, Choudary L. Bioactive compound analysis and antioxidant activity of endophytic bacterial extract from Adhathoda beddomei[J]. Asian Journal of Pharmaceutical and Clinical Research, 2015, 8(1): 70-72.
[14] Kloepper J W. A review of issues related to measuring colonization of plant roots by bacterial[J]. Canadian Journal of Microbiology, 1992, 38(6): 667-672.
[15] Hallmann J, Quadt-Hallmann A, Mahaffee W, et al. Bacterial endophytes in agricultural crop[J]. Canadian Journal of Microbiology, 1997, 43(10): 895-914.
[16] 畅涛, 王涵琦, 杨成德, 等. 马铃薯坏疽病Phoma foveata生防菌的筛选及鉴定[J]. 中国生物防治学报, 2014, 30(2): 247-252.
[17] 方中达. 植病研究方法(第三版)[M]. 北京: 中国农业出版社, 1997.
[18] 胡开辉, 罗庆国, 汪世华, 等. 化感水稻根际微生物类群及酶活性变化[J]. 应用生态学报, 2006, 17(6): 1060-1064.
[19] 杨洪凤, 余向阳, 薛雅蓉, 等. 内生解淀粉芽孢杆菌CC09在小麦根部定殖的电镜观察及防病效果[J]. 中国生物防治学报, 2014, 30(6): 839-844.
[20] 刘忠梅, 王霞, 赵金焕, 等. 有益内生菌B946在小麦体内的定殖规律[J]. 中国生物防治, 2005, 21(2): 113-116.
[21] Kus'mina L, Melent'ev A. Seed bacterization and rhizosphere of wheat seedings colonization by Bacillus cohn[J]. Mikrobiologiia, 2003, 72(2): 268-274.
[22] 陈美军, 段增强, 林先贵. 中国土壤质量标准研究现状与展望[J]. 土壤学报, 2011, 48(5): 1059-1071.
[23] 武佳蕊, 王宏伟, 谢星光, 等. 植物内生菌影响土壤微生物区系的研究进展[J]. 中国生态农业学报, 2014, 22(11): 1259-1266.
[24] 李轶, 唐佳妮, 吕绪凤, 等. 施用沼肥对设施土壤真菌动态变化的影响[J]. 中国沼气, 2013, 31(5): 29-39.
[25] Hannula S E, Wietsede B, Johannesvan V. A 3-year study reveals that plant growth stage, season and field site affect soil fungal communities while cultivar and GM-trait have minor effects[J]. PLoS ONE, 2012, 7(4): 1-13.
[26] 余贤美, 侯长明, 王海荣, 等. 枯草芽孢杆菌Bs-15在枣树体内和土壤中的定殖及其对土壤微生物多样性的影响[J]. 中国生物防治学报, 2014, 30(4): 497-502.
[27] 曹群, 丁文娟, 赵兰凤, 等. 生物有机肥对冬瓜枯萎病及土壤微生物和酶活性的影响[J]. 华南农业大学学报, 2015, 36(2): 36-42.