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journal1 ›› 2016, Vol. 32 ›› Issue (3): 372-378.DOI: 10.16409/j.cnki.2095-039x.2016.03.013

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Colonization of Antagonistic Endophytic Bacteria Bacillus mojavensis ZA1 in the Root of Potato and Rhizosphere Soil and Its Effect on Soil Microorganism

Wang Ying, Yang Chengde, Xue Li, Cui Yuezhen, Yang Xiaoli   

  1. College of Pratacultural Science, Gansu Agricultural University/Key Laboratory of Grassland Ecosystem, Ministry of Education/Pratacultural Engineering Laboratory of Gansu Province/Sino-U.S.A Center for Grazingland Ecosystem Sustainability, Lanzhou 730070, China
  • Received:2015-10-23 Online:2016-06-08 Published:2016-06-07

Abstract: GFP labelling technique and plate culture method were used to study the colonization of endophytic bacterium Bacillus mojavensis ZA1 in the root of potato and rhizosphere soil and its effect on soil microorganism. The results showed that ZA1-gfp colonized well in soil with a density of 3.7×104 to 7.3×104 cfu/g and colonized in the roots of potato and rhizosphere with densities of 49 to 630 cfu/g and 3.0×103 to 1.5×105 cfu/g, respectively. ZA1-gfp could be detected in inner roots and on the root hairs and root epidermis of potato under a fluorescence microscope. The number of soil bacteria and actinomycetes were increased significantly (P<0.05) after ZA1-gfp inoculation using root-drenching method, but fungi was decreased in 25 days later after ZA1-gfp inoculation; the number of soil bacteria, fungi and actinomycetes was kept well (There was no obvious difference). The results showed that ZA1-gfp could steadily colonize the roots of potato and rhizosphere and the community stability of soil microorganisms was kept well.

Key words: antagonistic endophytic bacteria, GFP labeled, potato, colonization dynamics, soil microorganism

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