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Chinese Journal Of Biological Control ›› 2020, Vol. 36 ›› Issue (2): 280-287.DOI: 10.16409/j.cnki.2095-039x.2020.02.014

• SCIENTIFIC NOTES • Previous Articles     Next Articles

Biological Control of Potato Verticillium Wilt by Microbial Fungicide

LU Xiuyun1, ZHAO Weisong1, BAI Ying2, MA Li3, ZHANG Lihong4, SHANG Junyan1, SU Zhenhe1, GUO Qinggang1, ZHANG Xiaoyun1, WANG Peipei1, YAN Lei1, MA Ping1, LI Shezeng1   

  1. 1. Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences/IPM Center of Hebei Province/Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture, Baoding 071000, China;
    2. Plant Protection and Quarantine Station of Baoding City, Baoding 071000, China;
    3. Laiyuan County Agricultural Bureau, Baoding 074300, China;
    4. Dabeiwang Agricultural Extesion Regional Station, Yongnian District, Handan City, Handan 057152, China
  • Received:2019-05-16 Online:2020-04-08 Published:2020-04-16

Abstract: Verticillium wilt is one of the important and widespread diseases in most potato regions of the world.It is a soil-borne and seed-borne vascular disease and difficult to control. Microbial fungicides have been confirmed as an effective measure to suppress crop soil-borne diseases. 109 spores/g Bacillus subtilis WP with the spores of B. subtilis NCD-2 as active ingredient was a microbial fungicides against crop soil-borne diseases. The safety of this preparation on potato seedling was evaluated by pot experiment, the method and dosage to control potato Verticillium wilt were investigated by field plot experiment and demonstrated in major potato production areas of Hebei Province. Pot experiment showed that seed-dressing with this microbial fungicide preparation was safe on potato emergence, which had no adverse effects on potato growth. Field plot experiment demonstrated that this preparation could significantly decrease potato Verticillium wilt and increase potato yields. In Weichang County, the treatments of seed-dressing with 30 kg/hm2 and seed-dressing with 30 kg/hm2 plus dripping irrigation with 15 kg/hm2 showed significant control efficacies against potato Verticillium wilt, respectively, compared to the control, meanwhile significantly increased the potato yield by 15.53% and 17.10%. In Xinle City, the treatment of seed-dressing with 30 kg/hm2 could significantly increase potato yield by 16.38%. The results from the demonstration fields showed that the treatment of seed-dressing with 30 kg/hm2 could significantly decrease potato Verticillium wilt by 84.22% and 72.93% in Laiyuan County and Weichang County, respectively, meanwhile increased yields by 24.30% and 9.27% respectively, compared to control, and significantly increase the yield by 19.73% compared to chemical control in Yongnian District. The results indicated that 109 spores/g B. subtilis WP was an excellent microbial preparation for potato Verticillium wilt control and potato yield increase, which provided the scientific basis for further application of this preparation in potato production.

Key words: potato Verticillium wilt, Bacillus subtilis, Verticillium dahliae, biological control

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