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journal1 ›› 2016, Vol. 32 ›› Issue (6): 794-799.DOI: 10.16409/j.cnki.2095-039x.2016.06.018

• SCIENTIFIC NOTES • Previous Articles     Next Articles

Influence of Cry8E Sublethal Doses on Activities of Detoxification Enzymes and Protective Enzyme of the Potato Beetle Larvae

REN Yu1, GUO Wenchao2,3, YUE Mingcui4, ABDUL Reheman·Tursun1   

  1. 1. College of Life and Geographical Science, Kashi University/Key Laboratory of Ecology and Biological Resources in Yarkand Oasis, Kashi 844000, China;
    2. Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences/Key Laboratory of Intergraded Management of Harmful Crop Vermin of China North-western Oasis, Ministry of Agriculture, Urumqi 830091, China;
    3. Institute of Microbiology, Urumqi 830091, China;
    4. State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
  • Received:2015-12-07 Online:2016-12-08 Published:2016-12-07

Abstract: In this paper, impact of Bacillus thuringiensis insecticidal crystal protein Cry8E to the activities of detoxification enzymes such as CarE, AChE, GST and of the catalase (CAT) in the midgut of the potato beetle larvae was investigated. The 2nd, 3rd and 4th instar larvae of the beetle were fed with the Cry8E insecticidal crystal protein at concentrations of 9 and 7 μg/mL. Then the activities of CarE, AChE, GST and CAT in the larvae were measured by the spectrophotometry. The LC50 was 11.3 μg/mL against the 1st instar larvae of the beetle. CarE activity increased significantly in treated insects than in the control and differed significantly between the two concentration treatments. And CarE activity of the 2nd instar larvae was different from that of the 4th instar larvae. The AChE activity in the 2nd instar larvae was higher in the treated insects than in the control, but was not different between the two concentration treatments. And AChE activity of the 2nd instar larvae differed significantly from that of the 3rd and 4th instars. GST activity was significantly lower in the treatments than in the control and differed significantly between the 2nd and the 3rd, 4th instar larvae. CAT activity increased and then decreased, and differed between the treatments and the control. And CAT activity also differed between the 3rd and 2nd, 4th instar larvae. These results show that the potato beetle can adapt to the impacts caused by the Cry8E insecticidal crystal proteins through changing activities of the detoxification enzymes and protective enzymes, which is influenced by the larval stage and protein concentration.

Key words: Bacillus thuringiensis, potato colorado beetle, caboxylesterase (CarE), acetylcholinesterase (AChE), glutathione-S-transferase (GST), catalase (CAT)

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