Influence of Cry8E Sublethal Doses on Activities of Detoxification Enzymes and Protective Enzyme of the Potato Beetle Larvae

doi:10.16409/j.cnki.2095-039x.2016.06.018

Abstract

Abstract: In this paper, impact of Bacillus thuringiensis insecticidal crystal protein Cry8E to the activities of detoxification enzymes such as CarE, AChE, GST and of the catalase (CAT) in the midgut of the potato beetle larvae was investigated. The 2nd, 3rd and 4th instar larvae of the beetle were fed with the Cry8E insecticidal crystal protein at concentrations of 9 and 7 μg/mL. Then the activities of CarE, AChE, GST and CAT in the larvae were measured by the spectrophotometry. The LC₅₀ was 11.3 μg/mL against the 1st instar larvae of the beetle. CarE activity increased significantly in treated insects than in the control and differed significantly between the two concentration treatments. And CarE activity of the 2nd instar larvae was different from that of the 4th instar larvae. The AChE activity in the 2nd instar larvae was higher in the treated insects than in the control, but was not different between the two concentration treatments. And AChE activity of the 2nd instar larvae differed significantly from that of the 3rd and 4th instars. GST activity was significantly lower in the treatments than in the control and differed significantly between the 2nd and the 3rd, 4th instar larvae. CAT activity increased and then decreased, and differed between the treatments and the control. And CAT activity also differed between the 3rd and 2nd, 4th instar larvae. These results show that the potato beetle can adapt to the impacts caused by the Cry8E insecticidal crystal proteins through changing activities of the detoxification enzymes and protective enzymes, which is influenced by the larval stage and protein concentration.

Key words: Bacillus thuringiensis, potato colorado beetle, caboxylesterase (CarE), acetylcholinesterase (AChE), glutathione-S-transferase (GST), catalase (CAT)

CLC Number:

S476.11

REN Yu, GUO Wenchao, YUE Mingcui, ABDUL Reheman·Tursun. Influence of Cry8E Sublethal Doses on Activities of Detoxification Enzymes and Protective Enzyme of the Potato Beetle Larvae[J]. journal1, 2016, 32(6): 794-799.

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