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journal1 ›› 2016, Vol. 32 ›› Issue (4): 503-510.DOI: 10.16409/j.cnki.2095-039x.2016.04.012

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Ferment Optimization of Biocontrol Fungus Snef5 against Meloidogyne incognita by Response Surface Methodology

YANG Chuanxu1, ZHAO Di4, TAN Zhuo1, ZHU Xiaofeng1, LIU Xiaoyu3, DUAN Yuxi1, WANG Yuanyuan2, CHEN Lijie1   

  1. 1. College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China;
    2. College of Biological Science and Technology, Shenyang Agricultural University, Shenyang 110866, China;
    3. College of Science, Shenyang Agricultural University, Shenyang 110866, China;
    4. Analytical Instrumentation Center, Shenyang Agricultural University, Shenyang 110866, China
  • Received:2016-01-21 Online:2016-08-08 Published:2016-07-29

Abstract: Simplicillium chinense strain Snef5 was screened as unique biological control fungi to plant nematodes in our previous research. In order to enhance its nematicidal activity, optimization of fermentation conditions were investigated for this fungus using response surface methodology. First, through Plackett-Burman screening experiments, three factors including the initial pH, the concentration of sucrose and MgSO4·7H2O in the broth appeared to have significant effects on the fermentation. According to the results of Box-Benhnken design and response surface analysis, the optimal conditions for strain Snef5 fermentation were obtained with the initial pH 6.74, 4.19% sucrose and 0.04% MgSO4·7H2O in the medium. Fermentation experiments with shake flasks and 30 L fermentor verified that there was no statistical difference between the nematicidal activity forecasted by the model and that obtained in the tests. After optimizing the fermentation broth, Snef5 enhanced its nematicidal activity by exhibition of increasing the second juvenile (J2) mortality rate of Meloidogyne incognita. Compared to the original fermentation broth, it showed 20.6% enhancement in the J2 mortality rate when using optimized broth in the flasks and 21.3% when using in the 30 L cylinders, respectively. Our research should have important implications for biocontrol fungi fermentation and application.

Key words: biocontrol fungus Simplicillium chinense Snef5, response surface methodology, Meloidogyne incognita, fermentation optimization

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