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Chinese Journal Of Biological Control ›› 2020, Vol. 36 ›› Issue (1): 113-124.DOI: 10.16409/j.cnki.2095-039x.2020.01.007

• RESEARCH REPORTS • Previous Articles     Next Articles

Optimization of the Fermentation Conditions of Biocontrol Agent Trichoderma longibrachiatum T6 and Its Efficiency against Pepper Blight

JING Fang1,2, ZHANG Shuwu1, LIU Jia1, XU Bingliang1   

  1. 1. College of Plant Protection, Gansu Agricultural University/Gansu Province Crop Disease and Pests Biological Control Enginnering Laboratory, Lanzhou 730070, China;
    2. Dingxi Academy of Agricultural Sciences, Dingxi 743000, China
  • Received:2019-01-17 Online:2020-02-08 Published:2020-02-26

Abstract: To optimize solid-state fermentation conditions for biocontrol agent Trichoderma longibrachiatum T6, the agricultural recycled waste by product grass charcoal, vermiculite, cow dung were used as the main medium components and measuring and optimizing the number of spores production of Trichoderma longibrachiatum T6 biocontrol agent by single factor tests and response surface methodology. The effects of moisture content, inoculation amount and air permeability on growth of the strain T6 were analyzed comprehensively. Subsequently, the effects of fermentation products on pepper growth and their biocontrol efficiency against pepper blight in pot experiment were detected, respectively. Our results of single factor tests demonstrated that the optimal fermentation conditions were moisture content of 60%, inoculation amount of 30%, air permeability is 9 layer gauze, temperature 28℃, pH 5.6. The results of comprehensive comparison showed that the effects of moisture content, inoculation amount and breathability on the spores production of Trichoderma longibrachiatum T6 were significant among the five factors. The optimum fermentation conditions were obtained by response surface analysis that moisture content of 64%, inoculation amount of 27%, air permeability is 9 layer gauze. Under these optimal conditions, the highest sporulation of Trichoderma longibrachiatum T6 could be 8.80841×108 cfu/g. Importantly, the treatment of pepper with soil and T. longibrachiatum T6 biocontrol agent 50:1 showed a strong effect of promoting the growth. Compared with control 1 (without any fertilizer), the relative growth rates of plant height, the ground fresh weight, root fresh weight, base stem diameter and root length were 54.90%, 82.46%, 89.19%, 56.41% and 46.62%, respectively. Finally, in the pot experiment, the pepper treated with T6 was thick and tall, with dark green leaves and developed roots. At the same time, the treatment of pepper with T. longibrachiatum T6 biocontrol agent could significantly control the disease spread and the control efficiency against pepper blight could be 54.8%, which was increased by 12.5% of the treatment with the chemicai agent carbendazol using the same treatment methods.

Key words: Trichoderma spp., response surface methodology, solid-state fermentation, blight, control efficiency

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