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journal1 ›› 2016, Vol. 32 ›› Issue (6): 712-720.DOI: 10.16409/j.cnki.2095-039x.2016.06.006

• RESEARCH REPORTS • Previous Articles     Next Articles

Cloning and Functional Analysis the PxAdoR Gene of Plutella xylostella Adenosine Receptor

FANG Ming1,2, CHAI Yiqiu1,2, CHEN Guanju2, ZHANG Jinming3, LÜ Yaobin3, HUANG Bo1   

  1. 1. Provincial Key Laboratory of Microbial Control, Anhui Agricultural University, Hefei 230036, China;
    2. Zhejiang Institute of Subtropical Crops/Key Laboratory of Entomogenous Fungi Resources Research and Development of Wenzhou, Wenzhou 325005, China;
    3. Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
  • Received:2016-02-04 Online:2016-12-08 Published:2016-12-07

Abstract: Differentially expressed fragments of Plutella xylostella treated with the metabolite N6-(2-hydroxyethyl)-adenosine (HEA) of Ophiocordyceps sobolifera were cloned using RT-PCR and RACE and a whole sequence (1828 bp) of adenosine receptor (PxAdoR) gene was obtained. The protein structure predicted and analyzed using the bioinformatics software is of seven transmembrane helices that belong to the G protein coupled receptors. The protein is hydrophobic and instable, without signal peptide, and of molecular weight of 49.2 kD. To make sure the PxAdoR protein functions, P. xylostella 2nd instar larvae were treated with HEA and the antagonist SCH58261 of mammal adenosine A2a receptor. Corrected mortality of treated P. xylostella larvae was significantly reduced by about 50% at 36 to 60 h. And the rate of weight growth inhibition was reduced significantly from 43.3%, 38.3% and 46.2% to 13.2%, 10.3% and 8.6% at 36, 48 and 60 h, respectively. It is prospective that HEA may act by PxAdoR and PxAdoR may be a new insecticidal target for pest control.

Key words: adenosine receptors, gene clone, adenosine receptor antagonist, protein structure, N6-(2-hydroxyethyl)-adenosine

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