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journal1 ›› 2017, Vol. 33 ›› Issue (1): 142-150.DOI: 10.16409/j.cnki.2095-039x.2017.01.020

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Identification of Bacillus atrophaeus YL3 and Detection of Its Lipopeptide Compounds

LIU Youzhou1, CHEN Xijun2, LIANG Xuejie1, QIAN Yaming3, QIAO Junqing1, LIU Yongfeng1   

  1. 1. Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
    2. College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 220009, China;
    3. Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
  • Received:2016-05-26 Online:2017-02-08 Published:2017-01-24

Abstract: In order to investigate the biological characteristics and evaluate the antagonistic activity of the strain YL3, the biochemical characteristics, 16S rDNA and gyrB sequences were used to identify the species. The antagonistic activity of strain YL3 in vitro and control effect on strawberry fusarium wilt in field were investigated in this study. The selective medium and crystal violet staining method were used to detect the extracellular enzymes and biofilm formation of the strain YL3. The lipopeptide compounds produced by strain YL3 was extracted by acid precipitation method and analyzed by LC-MS. The results showed that strain YL3 was identified as Bacillus atrophaeus. Strain YL3 had strong antagonistic activities against the growth of pathogenic fungi such as Fusarium oxysporum, Colletotrichum fragariae, Alternaria brassicae, Sclerotinia sclerotiorumErwinia amylovora, Xanthomonas oryzae pv. oryzicola and Bacillus megaterium. The 10 times dilution and 100 times dilution of strain YL3 fermented liquid could effectively control fusarium wilt on strawberry, "Ningyu" with the control efficiency of 82.86% and 67.62%, respectively. Strain YL3 had the ability to produce proteases, chitinases and siderophores, and form and retain a thick biofilm on the well wall after culturing for 3 days. The lipopeptide compounds produced by strain YL3 could inhibit more effectively the mycelia growth of Fusarium oxysporum along with the dosage increased. Surfactins and fengycins were detected from the lipopeptide compounds of strain YL3 by LC-MS. Therefore, the strain YL3 was a prospective antagonistic bacterium.

Key words: Bacillus atrophaeus, strawberry fusarium wilt, lipopeptide compounds

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