Enhanced Tolerance of Tomatoes against Fusarium oxysporum by Inoculation with Dark Septate Endophyte

doi:10.16409/j.cnki.2095-039x.2017.03.014

Abstract

Abstract: Tolerance of tomato plants (Lycopersicon esculentum) against fungal pathogen Fusarium oxysporum was investigated by inoculation with two biocontrol agents, dark septate endophyte (DSE) strains K36 and Z48 of Phialophora mustea, and the influence on antioxidant enzymes was also assayed. Under the treatment of F. oxysporum on tomato plants, the inoculation of two DSE strains significantly alleviated the growth inhibition of tomatoes, compared with the uninoculated-DSE controls. Inoculation of the strains K36 and Z48 significantly increased plant height by 46.4% and 53.2%, and enhanced tomato shoot and root biomasses by 60.6% and 50%, 63.7% and 65.9%, respectively. Moreover, DSE inoculation further triggered the antioxidant response of tomatoes, and increased the activities of SOD by 19.3% and 8.1%, POD by 14.0% and 4.4%, respectively. The enhanced antioxidant enzymes resulted in the alleviated cell-induced lipid peroxidation stress caused by fungal pathogens, compared with the uninoculated DSE treatments under F. oxysporum inoculation. Our results revealed that DSE colonization could improve the plant resistance against wilt disease caused by F. oxysporum and further promote the growth of host plants.

Key words: dark septate endophytes, Phialophora mustea, Lycopersicon esculentum, disease resistance, Fusarium oxysporum, antioxidant enzymes

CLC Number:

S476

ZHANG Xiaorong, LI Tao, WANG Chaojun, ZHU Lingling, XU Lujuan, ZHAO Zhiwei. Enhanced Tolerance of Tomatoes against Fusarium oxysporum by Inoculation with Dark Septate Endophyte[J]. journal1, 2017, 33(2/3): 394-400.

References

[1] Smith D L, Subramanian S, Lamont J R, et al. Signaling in the phytomicrobiome: breadth and potential[J]. Frontiers in Plant Science, 2015, 6(1): 709.
[2] Quiza L, St-Arnaud M, Yergeau E. Harnessing phytomicrobiome signaling for rhizosphere microbiome engineering[J]. Frontiers in Plant Science, 2015, 6(1): 507.
[3] Pérez L I, Gundel P E, Omacini M. Can the defensive mutualism between grasses and fungal endophytes protect non-symbiotic neighbours from soil pathogens?[J]. Plant and Soil, 2016, 405(1): 289-298.
[4] Jumpponen A R I, Trappe J M. Dark septate endophytes: a review of facultative biotrophic root-colonizing fungi[J]. New Phytologist, 1998, 140(2): 295-310.
[5] 刘茂军, 张兴涛, 赵之伟, 等. 深色有隔内生真菌(DSE)研究进展[J]. 菌物学报, 2009, 28(6): 888-894.
[6] 闫姣, 贺学礼, 张亚娟, 等. 荒漠北沙柳根系丛枝菌根真菌和黑隔内生真菌定殖状况[J]. 植物生态学报, 2014, 38(9): 949-958.
[7] Fracchia S, Krapovickas L, Aranda-Rickert A, et al. Dispersal of arbuscular mycorrhizal fungi and dark septate endophytes by Ctenomys cf. knighti (Rodentia) in the northern Monte Desert of Argentina[J]. Journal of Arid Environments, 2011, 75(11): 1016-1023.
[8] Mandyam K, Jumpponen A. Seeking the elusive function of the root-colonising dark septate endophytic fungi[J]. Studies in Mycology, 2005, 53(1): 173-189.
[9] Della Monica I F, Saparrat M C N, Godeas A M, et al. The co-existence between DSE and AMF symbionts affects plant P pools through P mineralization and solubilization processes[J]. Fungal Ecology, 2015, 17(1): 10-17.
[10] Newsham K K. A meta-analysis of plant responses to dark septate root endophytes[J]. New Phytologist, 2011, 190(3): 783-793.
[11] Barrow J R. Atypical morphology of dark septate fungal root endophytes of Bouteloua in arid southwestern USA rangelands[J]. Mycorrhiza, 2003, 13(5): 239-247.
[12] Addy H D, Piercey M M, Currah R S. Microfungal endophytes in roots[J]. Canadian Journal of Botany, 2005, 83(1): 1-13.
[13] Huusko K, Ruotsalainen A L, Markkola A M. A shift from arbuscular mycorrhizal to dark septate endophytic colonization in Deschampsia flexuosa roots occurs along primary successional gradient[J]. Mycorrhiza, 2017, 27(2): 129-138.
[14] Narisawa K, Usuki F, Hashiba T. Control of Verticillium yellows in Chinese cabbage by the dark septate endophytic fungus LtVB3[J]. Phytopathology, 2004, 94(5): 412-418.
[15] Khastini R O, Ohta H, Narisawa K. The role of a dark septate endophytic fungus, Veronaeopsis simplex Y34, in Fusarium disease suppression in Chinese cabbage[J]. Journal of Microbiology, 2012, 50(4): 618-624.
[16] Terhonen E, Sipari N, Asiegbu F O. Inhibition of phytopathogens by fungal root endophytes of Norway spruce[J]. Biological Control, 2016, 99(1): 53-63.
[17] Wang J, Li T, Liu G, et al. Unraveling the role of dark septate endophyte (DSE) colonizing maize (Zea mays) under cadmium stress: physiological, cytological and genic aspects[J]. Scientific Reports, 2016, 6(1): 22028.
[18] 李凯龙, 王艺潼, 韩晓雪, 等. 低钾胁迫对番茄叶片活性氧及抗氧化酶系的影响[J]. 西北植物学报, 2013, 33(1): 66-73.
[19] Berch S M, Kendrick B. Vesicular-arbuscular mycorrhizae of southern Ontario ferns and fern-allies[J]. Mycologia, 1982, 74(5): 769-776.
[20] Barrow J, Aaltonen R. Evaluation of the internal colonization of Atriplex canescens (Pursh) Nutt. roots by dark septate fungi and influence of host physiological activity[J]. Mycorrhiza, 2001, 11(4): 199-205.
[21] Xu R, Li T, Cui H, et al. Diversity and characterization of Cd-tolerant dark septate endophytes (DSEs) associated with the roots of Nepal alder (Alnus nepalensis) in a metal mine tailing of southwest China[J]. Applied Soil Ecology, 2015, 93(1): 11-18.
[22] Grünig C R, Sieber T N, Rogers S O, et al. Genetic variability among strains of Phialocephala fortinii and phylogenetic analysis of the genus Phialocephala based on rDNA ITS sequence comparisons[J]. Canadian Journal of Botany, 2002, 80(12): 1239-1249.
[23] 钱晓雍, 沈根祥, 黄丽华, 等. 3株非致病性镰刀菌(Fusarium oxysporum)菌株对番茄枯萎病的生物防治效果[J]. 上海农业学报, 2007, 23(4): 60-62.
[24] Tosi L, Zazzerini A. Phialophora mustea, a new lily pathogen[J]. Petria, 1996, 6(3): 209-214.
[25] Wani Z A, Mirza D N, Arora P, et al. Molecular phylogeny, diversity, community structure, and plant growth promoting properties of fungal endophytes associated with the corms of saffron plant: An insight into the microbiome of Crocus sativus Linn[J]. Fungal Biology, 2016, 120(12): 1509-1524.
[26] Berthelot C, Leyval C, Foulon J, et al. Plant growth promotion, metabolite production and metal tolerance of dark septate endophytes isolated from metal-polluted poplar phytomanagement sites[J]. FEMS Microbiology Ecology, 2016, 92(10): fiw144.
[27] Mandal S, Mitra A, Mallick N. Biochemical characterization of oxidative burst during interaction between Solanum lycopersicum and Fusarium oxysporum f. sp. lycopersici[J]. Physiological and Molecular Plant Pathology, 2008, 72(1): 56-61.
[28] Moreira B C, Junior P P, Jordão T C, et al. Effect of inoculation of symbiotic fungi on the growth and antioxidant enzymes' activities in the presence of Fusarium subglutinans f. sp. ananas in pineapple plantlets[J]. Acta Physiologiae Plantarum, 2016, 38(10): 235.
[29] Alberton O, Kuyper T W, Summerbell R C. Dark septate root endophytic fungi increase growth of Scots pine seedlings under elevated CO₂ through enhanced nitrogen use efficiency[J]. Plant and Soil, 2010, 328(1/2): 459-470.