Nanomaterials-dsRNA Prevent Tobacco Mosaic Virus Infection

doi:10.16409/j.cnki.2095-039x.2018.05.010

Abstract

Abstract: Application of exogenous double-stranded RNAs targeted to viral genes can induce RNAi in plants, but dsRNA is easy to be degraded and only effective in a limited time, which limits the application of this technology. In this study, TMV coat protein gene was used as the target fragment to synthesize dsRNA in vitro, and then fused with two kinds of nanomaterials, chitosan (CS) and layered double hydroxide (LDH) to synthesize CS-dsRNA and LDH-dsRNA preparations. The stability of the two preparations was tested, and the prevention and therapeutic effects on TMV were also investigated. The results showed that the amount of dsRNA decreased by 80.01%, 58.89% and 59.76%, respectively, at 4℃, 25℃ and 37℃ for 30 days. The degradation rate of dsRNA at 4℃ was slightly higher than that at 25℃ and 37℃. The amount of dsRNA in CS-dsRNA preparation increased by 65.98%, 103.82% and 157%, respectively, and that in LDH-dsRNA preparation increased by 54.30%, 58.4% and 66.42%, respectively. With the increase of temperature, the amount of dsRNA increased, indicating that the nanocomposite dsRNA has a slow release effect. The experiment results of half-leaf method showed that the preventive effects of dsRNA, CS-dsRNA and LDH-dsRNA on TMV were 60.12%, 57.79% and 55.18%, and the therapeutic effects were 65%, 58.4% and 59.08%, respectively. This study showed that the nano-materials (CS and LDH) play a good role in the protection, slow release and stability of dsRNA, and a combination of nano-materials and RNAi have a promising application in the prevention and treatment of plant viral diseases.

Key words: chitosan, LDH, dsRNA, tobacco mosaic virus, prevention

CLC Number:

S476

WANG Jiaqi, LIU Yong, ZHANG Songbai, ZHU Chunhui, SUN Shu'e, ZHANG Deyong. Nanomaterials-dsRNA Prevent Tobacco Mosaic Virus Infection[J]. journal1, 2018, 34(5): 715-721.

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