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Chinese Journal Of Biological Control ›› 2020, Vol. 36 ›› Issue (1): 135-144.DOI: 10.16409/j.cnki.2095-039x.2020.01.009

• RESEARCH REPORTS • Previous Articles     Next Articles

Studies on Antibacterial and Plant-Growth-Promoting Characterization in Biocontrol Bacterial Strains PA2101 and PG3402

QIAN Huimin1, ZHAO Hui1, LIU Xintao1, NI Yunxia1, QIU Rui2, LI Xiaojie2, ZHAO Xinbei1, LI Shujun2, LIU Hongyan1, WEN Yi1   

  1. 1. Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China;
    2. Tobacco Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory for Green Preservation & Control of Tobacco Diseases and Pests in Huanghuai Growing Area Tobacco, Xuchang 461000, China
  • Received:2019-04-11 Online:2020-02-08 Published:2020-02-26

Abstract: We investigated the antimicrobial activity, inhibition and plant-growth-promoting characterization of Pseudomonas aeruginosa PA2101 and Pseudomonas granadensis PG3402 with great biocontrol potential for tobacco in our laboratory. The results showed that PA2101 and PG3402 greatly inhibited Fusarium oxysporum, Alternaria alternata, Rhizoctonia cerealis, Macrophomina phaseolina and Phoma herbarum. The fermentation filtrates of the two bacteria caused the abnormal hyphal growth of Phytophthora nicotianae and Thielaviopsis basicola, as well as inhibited spore germination of T. basicola. They could produce volatile substances with inhibitory activity against P. nicotianae and T. basicola. Both bacteria were detected to be able to produce protease, cellulose and β-1,3-glucanase. Meanwhile, PA2101 was detected to be able to secrete chitinase. Both could synthesize HCN and harbored the genes for synthesis of phenazine-1-carboxylic acid (PCA). PG3402 had the genes for synthesis of 2,4-diacetylphloroglucinal (DAPG). It was found that PA2101 and PG3402 could produce siderophores and IAA, and that they had the characteristic of solubilizing phosphorus. To sum up, the two bacteria PA2101 and PG3402 have a broad antimicrobial spectrum, and can inhibit the growth of P. nicotianae and T. basicola. They can produce a variety of inhibition and plant growth promoting substances, which also contain specific antibiotic genes, indicating that PA2101 and PG3402 have multiple biocontrol functions and that they can be used for prevention of tobacco disease.

Key words: biocontrol bacteria, antimicrobial activity, inhibition characterization, plant-growth-promoting mechanism

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