Screening of Biocontrol Bacteria on Rice Bacteria Leaf Streak and Determination of Field Control Efficacy

doi:10.16409/j.cnki.2095-039x.2019.04.022

Abstract

Abstract: In order to screen better biocontrol bacteria against rice bacteria leaf streak (BLS) caused by Xanthomonas oryzae pv.oryzicola, the biocontrol efficacy of four biocontrol bacteria strains on rice bacteria leaf streak were evaluated by plate confrontation test, greenhouse and field experiments. In the plate confrontation test, the antibacterial activities of the four biocontrol bacteria strains were determined. Greenhouse experiment showed that except for biocontrol strain LH, the plant height, root length and fresh weight of the rice seedlings treated with the other three biocontrol bacteria fermentation broths were significantly higher than those of the control. Inoculation test indicated that application of biocontrol bacteria before inoculation of pathogenic X. oryzae had the best control effect. Field trials demonstrated that the biocontrol strain L1 achieved more than 55% control efficacy in three different fields, and the yield increase rate was more than 10%, which was significantly higher than other treatments. This study shows that the biocontrol strain L1 can not only be used for the biological control of the BLS disease, but also promote rice growth, thus it has a good potential application.

Key words: rice bacteria leaf streak, biocontrol bacteria, plant growth promotion, biological control

CLC Number:

S476

WANG Xing, YANG Jun, CAO Weihua, WEI Lanfang, JI Guanghai. Screening of Biocontrol Bacteria on Rice Bacteria Leaf Streak and Determination of Field Control Efficacy[J]. Chinese Journal Of Biological Control, 2019, 35(4): 648-654.

References

[1] Hummel A W, Wilkins K E, Wang L, et al. A transcription activator-like effector from Xanthomonas oryzae pv. oryzicola elicits dose-dependent resistance in rice[J]. Molecular Plant Pathology, 2016, 18(1):55-66.
[2] Niñoliu D O, Ronald P C, Bogdanove A J. Xanthomonas oryzae pathovars:model pathogens of a model crop[J]. Molecular Plant Pathology, 2010, 7(5):303-324.
[3] 周丽洪, 韩阳, 李淼, 等. 西南地区水稻细菌性条斑病菌链霉素抗性研究[J]. 云南农业大学学报, 2014, 29(5):654-660.
[4] Idris E S E, Iglesias D J, Talon M, et al. Tryptophan-dependent production of indole-3-acetic acid (IAA) affects level of plant growth promotion by Bacillus amyloliquefaciens FZB42[J]. Molecular Plant-Microbe Interactions, 2007, 20(6):619.
[5] 董国菊, 李文英, 刘翠平, 等. 烟草疫霉拮抗菌株P-72-10的鉴定及其拮抗代谢产物初步分析[J]. 植物病理学报, 2012, 42(3):297-305.
[6] 彭兵, 张树斌, 贾宇, 等. 枯草芽孢杆菌菌株A抗菌蛋白的分离纯化及抗真菌机理[J]. 中国农业科学, 2011, 44(1):67-74.
[7] Folman L B, Postma J, van Veen J A. Characterisation of Lysobacter enzymogenes (Christensen and Cook 1978) strain 3.1T8, a powerful antagonist of fungal diseases of cucumber[J]. Microbiological Research, 2003, 158(2):107-115.
[8] Kobayashi D Yuen G. The potential of Lysobacter spp. as bacterial biological control agents for plant diseases[J]. CAB Review:Perspectives in Agriculture Veterinary Science Nutrition and Natural Resources, 2007, 2(7):1-11.
[9] Luo C, Liu X, Zhou H, et al. Nonribosomal peptide synthase gene clusters for lipopeptide biosynthesis in Bacillus subtilis 916 and their phenotypic functions[J]. Applied and Environmental Microbiology, 2015, 81(1):422-431.
[10] 张淼, 周立梅, 徐德阳, 等. 侧孢短芽孢杆菌BL-21抗菌蛋白的稳定性分析和分离纯化[J]. 中国农学通报, 2017, 33(15):43-48.
[11] 李红晓, 张殿朋, 赵洪新, 等. 解淀粉芽胞杆菌MH71抗菌物质理化特性及对番茄灰霉病菌的抑菌活性[J]. 中国生物防治学报, 2016, 32(4):485-492.
[12] Zhang R S, Liu Y F, Luo C P, et al. Bacillus amyloliquefaciens Lx-11, a potential biocontrol agent against rice bacterial leaf streak[J]. Journal of Plant Pathology, 2012, 94(3):609-619.
[13] Jetiyanon K, Wei G, Tuzun S, et al. Induced systemic resistance (ISR) of cucumber by stem jection and seed treatment with PGPR[J]. Phytopathology, 1995, 85:114.
[14] 陈志谊, 许志刚, 陆凡, 等. 拮抗细菌B-916对水稻植株的抗性诱导作用[J]. 西南农业学报, 2001, 14(2):44-48.
[15] 李德全, 陈志谊, 聂亚锋. 生防菌Bs-916及高效突变菌株抗菌物质及其对水稻抗性诱导作用的研究[J]. 植物病理学报, 2008, 38(2):192-198.
[16] 陈思宇. 水稻纹枯病菌拮抗细菌的筛选与防治作用研究[D]. 南京:南京农业大学, 2013.
[17] 王卉, 尚庆茂, 张志刚, 等. 解淀粉芽孢杆菌L-H15产促生物质分析及发酵工艺优化[J]. 食品科学, 2017, 38(10):74-81.
[18] 闫志宇, 翟蓓蓓, 李术娜, 等. 乙草胺降解菌L3的促生物质[J]. 江苏农业科学, 2017, 45(22):295-298.
[19] 杨晓云, 陈志谊, 蒋盼盼, 等. 解淀粉芽孢杆菌B1619对番茄的促生作用[J]. 中国生物防治学报, 2016, 32(3):349-356.
[20] Hyunsun K, Jin R D, Krishnan H B, et al. Biocontrol ability of Lysobacter antibioticus HS124 against Phytophthora blight is mediated by the production of 4-hydroxyphenylacetic acid and several lytic enzymes[J]. Current Microbiology, 2009, 59(6):608-615.
[21] Lou L, Qian G, Xie Y, et al. Biosynthesis of HSAF, a tetramic acid-containing macrolactam from Lysobacter enzymogenes[J]. Journal of the American Chemical Society, 2011, 133(4):643-645.
[22] 王皓楠, 靳鹏飞, 康迅, 等. 解淀粉芽孢杆菌HAB-6抑菌物质及其相关基因的分析[J]. 江苏农业科学, 2018, 46(4):79-83.
[23] Zhao Y, Qian G, Ye Y, et al. Heterocyclic aromatic N-oxidation in the biosynthesis of phenazine antibiotics from Lysobacter antibioticus[J]. Organic Letters, 2016, 18(10):2495-2498.
[24] Laborda P, Zhao Y, Ling J, et al. Production of antifungal p-aminobenzoic acid in Lysobacter antibioticus OH13[J]. Journal of Agricultural and Food Chemistry, 2018, 66(3):630.
[25] Gardener M S, Kim I S, Kim K Y, et al. Draft genome sequence of a chitinase-producing biocontrol bacterium, Lysobacter antibioticus HS124[J]. Plant Disease, 2014, 20(3):216-218.